Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells

Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells

Human DICER1 protein cleaves double-stranded RNA into small sizes, a crucial step in production of single-stranded RNAs which are mediating factors of cytoplasmic RNA interference. Here, we clearly demonstrate that human DICER1 protein localizes not only to the cytoplasm but also to the nucleoplasm....

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Main Authors: Ando, Yoshinari, Tomaru, Yasuhiro, Morinaga, Ayako, Burroughs, Alexander Maxwell, Kawaji, Hideya, Kubosaki, Atsutaka, Kimura, Ryuichiro, Tagata, Maiko, Ino, Yoko, Hirano, Hisashi, Chiba, Joe, Suzuki, Harukazu, Carninci, Piero, Hayashizaki, Yoshihide
Format: Online
Language:English
Published: Public Library of Science 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156128/
id pubmed-3156128
recordtype oai_dc
spelling pubmed-31561282011-08-19 Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells Ando, Yoshinari Tomaru, Yasuhiro Morinaga, Ayako Burroughs, Alexander Maxwell Kawaji, Hideya Kubosaki, Atsutaka Kimura, Ryuichiro Tagata, Maiko Ino, Yoko Hirano, Hisashi Chiba, Joe Suzuki, Harukazu Carninci, Piero Hayashizaki, Yoshihide Research Article Human DICER1 protein cleaves double-stranded RNA into small sizes, a crucial step in production of single-stranded RNAs which are mediating factors of cytoplasmic RNA interference. Here, we clearly demonstrate that human DICER1 protein localizes not only to the cytoplasm but also to the nucleoplasm. We also find that human DICER1 protein associates with the NUP153 protein, one component of the nuclear pore complex. This association is detected predominantly in the cytoplasm but is also clearly distinguishable at the nuclear periphery. Additional characterization of the NUP153-DICER1 association suggests NUP153 plays a crucial role in the nuclear localization of the DICER1 protein. Public Library of Science 2011-08-15 /pmc/articles/PMC3156128/ /pubmed/21858095 http://dx.doi.org/10.1371/journal.pone.0023385 Text en Ando et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Ando, Yoshinari
Tomaru, Yasuhiro
Morinaga, Ayako
Burroughs, Alexander Maxwell
Kawaji, Hideya
Kubosaki, Atsutaka
Kimura, Ryuichiro
Tagata, Maiko
Ino, Yoko
Hirano, Hisashi
Chiba, Joe
Suzuki, Harukazu
Carninci, Piero
Hayashizaki, Yoshihide
spellingShingle Ando, Yoshinari
Tomaru, Yasuhiro
Morinaga, Ayako
Burroughs, Alexander Maxwell
Kawaji, Hideya
Kubosaki, Atsutaka
Kimura, Ryuichiro
Tagata, Maiko
Ino, Yoko
Hirano, Hisashi
Chiba, Joe
Suzuki, Harukazu
Carninci, Piero
Hayashizaki, Yoshihide
Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells
author_facet Ando, Yoshinari
Tomaru, Yasuhiro
Morinaga, Ayako
Burroughs, Alexander Maxwell
Kawaji, Hideya
Kubosaki, Atsutaka
Kimura, Ryuichiro
Tagata, Maiko
Ino, Yoko
Hirano, Hisashi
Chiba, Joe
Suzuki, Harukazu
Carninci, Piero
Hayashizaki, Yoshihide
author_sort Ando, Yoshinari
title Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells
title_short Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells
title_full Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells
title_fullStr Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells
title_full_unstemmed Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells
title_sort nuclear pore complex protein mediated nuclear localization of dicer protein in human cells
description Human DICER1 protein cleaves double-stranded RNA into small sizes, a crucial step in production of single-stranded RNAs which are mediating factors of cytoplasmic RNA interference. Here, we clearly demonstrate that human DICER1 protein localizes not only to the cytoplasm but also to the nucleoplasm. We also find that human DICER1 protein associates with the NUP153 protein, one component of the nuclear pore complex. This association is detected predominantly in the cytoplasm but is also clearly distinguishable at the nuclear periphery. Additional characterization of the NUP153-DICER1 association suggests NUP153 plays a crucial role in the nuclear localization of the DICER1 protein.
publisher Public Library of Science
publishDate 2011
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156128/
_version_ 1611470897234837504

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