The role of the precursor structure in the biogenesis of microRNA

The role of the precursor structure in the biogenesis of microRNA

The human genome contains more than 1,000 microRNA (miRNA) genes, which are transcribed mainly by RNA polymerase II. The canonical pathway of miRNA biogenesis includes the nuclear processing of primary transcripts (pri-miRNAs) by the ribonuclease Drosha and further cytoplasmic processing of pre-miRN...

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Main Authors: Starega-Roslan, Julia, Koscianska, Edyta, Kozlowski, Piotr, Krzyzosiak, Wlodzimierz J.
Format: Online
Language:English
Published: SP Birkhäuser Verlag Basel 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3155042/
id pubmed-3155042
recordtype oai_dc
spelling pubmed-31550422011-09-21 The role of the precursor structure in the biogenesis of microRNA Starega-Roslan, Julia Koscianska, Edyta Kozlowski, Piotr Krzyzosiak, Wlodzimierz J. Review The human genome contains more than 1,000 microRNA (miRNA) genes, which are transcribed mainly by RNA polymerase II. The canonical pathway of miRNA biogenesis includes the nuclear processing of primary transcripts (pri-miRNAs) by the ribonuclease Drosha and further cytoplasmic processing of pre-miRNAs by the ribonuclease Dicer. This review discusses the issue of miRNA end heterogeneity generated primarily by Drosha and Dicer cleavage and focuses on the structural aspects of the Dicer step of miRNA biogenesis. We examine the structures of miRNA precursors, both predicted and experimentally determined, as well as the influence of various motifs that disturb the regularity of pre-miRNA structure on Dicer cleavage specificity. We evaluate the structural determinants of the length diversity of miRNA generated by Dicer from different precursors and highlight the importance of asymmetrical motifs. Finally, we discuss the impact of Dicer protein partners on cleavage efficiency and specificity and propose the contribution of pre-miRNA structural plasticity to the dynamics of the dicing complex. SP Birkhäuser Verlag Basel 2011-05-24 2011-09 /pmc/articles/PMC3155042/ /pubmed/21607569 http://dx.doi.org/10.1007/s00018-011-0726-2 Text en © The Author(s) 2011
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Starega-Roslan, Julia
Koscianska, Edyta
Kozlowski, Piotr
Krzyzosiak, Wlodzimierz J.
spellingShingle Starega-Roslan, Julia
Koscianska, Edyta
Kozlowski, Piotr
Krzyzosiak, Wlodzimierz J.
The role of the precursor structure in the biogenesis of microRNA
author_facet Starega-Roslan, Julia
Koscianska, Edyta
Kozlowski, Piotr
Krzyzosiak, Wlodzimierz J.
author_sort Starega-Roslan, Julia
title The role of the precursor structure in the biogenesis of microRNA
title_short The role of the precursor structure in the biogenesis of microRNA
title_full The role of the precursor structure in the biogenesis of microRNA
title_fullStr The role of the precursor structure in the biogenesis of microRNA
title_full_unstemmed The role of the precursor structure in the biogenesis of microRNA
title_sort role of the precursor structure in the biogenesis of microrna
description The human genome contains more than 1,000 microRNA (miRNA) genes, which are transcribed mainly by RNA polymerase II. The canonical pathway of miRNA biogenesis includes the nuclear processing of primary transcripts (pri-miRNAs) by the ribonuclease Drosha and further cytoplasmic processing of pre-miRNAs by the ribonuclease Dicer. This review discusses the issue of miRNA end heterogeneity generated primarily by Drosha and Dicer cleavage and focuses on the structural aspects of the Dicer step of miRNA biogenesis. We examine the structures of miRNA precursors, both predicted and experimentally determined, as well as the influence of various motifs that disturb the regularity of pre-miRNA structure on Dicer cleavage specificity. We evaluate the structural determinants of the length diversity of miRNA generated by Dicer from different precursors and highlight the importance of asymmetrical motifs. Finally, we discuss the impact of Dicer protein partners on cleavage efficiency and specificity and propose the contribution of pre-miRNA structural plasticity to the dynamics of the dicing complex.
publisher SP Birkhäuser Verlag Basel
publishDate 2011
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3155042/
_version_ 1611470604974686208

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