PACAP centrally mediates emotional stress-induced corticosterone responses in mice
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide widely distributed in the nervous system. Recently, PACAP was shown to be involved in restraint stress-induced corticosterone release and concomitant expression of the genes involved in hypothalamic–pituitary–ad...
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Informa Healthcare
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128825/ |
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pubmed-31288252011-07-12 PACAP centrally mediates emotional stress-induced corticosterone responses in mice Tsukiyama, Naohiro Saida, Yoko Kakuda, Michiya Shintani, Norihito Hayata, Atsuko Morita, Yoshiko Tanida, Mamoru Tajiri, Minako Hazama, Keisuke Ogata, Katsuya Hashimoto, Hitoshi Baba, Akemichi Original Article Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide widely distributed in the nervous system. Recently, PACAP was shown to be involved in restraint stress-induced corticosterone release and concomitant expression of the genes involved in hypothalamic–pituitary–adrenal (HPA) axis activation. Therefore, in this study, we have addressed the types of stressors and the levels of the HPA axis in which PACAP signaling is involved using mice lacking PACAP (PACAP−/−). Among four different types of stressors, open-field exposure, cold exposure, ether inhalation, and restraint, the corticosterone response to open-field exposure and restraint, which are categorized as emotional stressors, but not the other two, was markedly attenuated in PACAP−/− mice. Peripheral administration of corticotropin releasing factor (CRF) or adrenocorticotropic hormone induced corticosterone increase similarly in PACAP and wild-type mice. In addition, the restraint stress-induced c-Fos expression was significantly decreased in the paraventricular nucleus (PVN) and medial amygdala (MeA), but not the medial prefrontal cortex, in PACAP−/− mice. In the PVN of PACAP−/− mice, the stress-induced c-Fos expression was blunted in the CRF neurons. These results suggest that PACAP is critically involved in activation of the MeA and PVN CRF neurons to centrally regulate the HPA axis response to emotional stressors. Informa Healthcare 2011-07 2011-03-27 /pmc/articles/PMC3128825/ /pubmed/21438773 http://dx.doi.org/10.3109/10253890.2010.544345 Text en © 2011 Informa Healthcare http://creativecommons.org/licenses/by/2.0/ This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Informa Healthcare journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Tsukiyama, Naohiro Saida, Yoko Kakuda, Michiya Shintani, Norihito Hayata, Atsuko Morita, Yoshiko Tanida, Mamoru Tajiri, Minako Hazama, Keisuke Ogata, Katsuya Hashimoto, Hitoshi Baba, Akemichi |
| spellingShingle |
Tsukiyama, Naohiro Saida, Yoko Kakuda, Michiya Shintani, Norihito Hayata, Atsuko Morita, Yoshiko Tanida, Mamoru Tajiri, Minako Hazama, Keisuke Ogata, Katsuya Hashimoto, Hitoshi Baba, Akemichi PACAP centrally mediates emotional stress-induced corticosterone responses in mice |
| author_facet |
Tsukiyama, Naohiro Saida, Yoko Kakuda, Michiya Shintani, Norihito Hayata, Atsuko Morita, Yoshiko Tanida, Mamoru Tajiri, Minako Hazama, Keisuke Ogata, Katsuya Hashimoto, Hitoshi Baba, Akemichi |
| author_sort |
Tsukiyama, Naohiro |
| title |
PACAP centrally mediates emotional stress-induced corticosterone responses in mice |
| title_short |
PACAP centrally mediates emotional stress-induced corticosterone responses in mice |
| title_full |
PACAP centrally mediates emotional stress-induced corticosterone responses in mice |
| title_fullStr |
PACAP centrally mediates emotional stress-induced corticosterone responses in mice |
| title_full_unstemmed |
PACAP centrally mediates emotional stress-induced corticosterone responses in mice |
| title_sort |
pacap centrally mediates emotional stress-induced corticosterone responses in mice |
| description |
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide widely distributed in the nervous system. Recently, PACAP was shown to be involved in restraint stress-induced corticosterone release and concomitant expression of the genes involved in hypothalamic–pituitary–adrenal (HPA) axis activation. Therefore, in this study, we have addressed the types of stressors and the levels of the HPA axis in which PACAP signaling is involved using mice lacking PACAP (PACAP−/−). Among four different types of stressors, open-field exposure, cold exposure, ether inhalation, and restraint, the corticosterone response to open-field exposure and restraint, which are categorized as emotional stressors, but not the other two, was markedly attenuated in PACAP−/− mice. Peripheral administration of corticotropin releasing factor (CRF) or adrenocorticotropic hormone induced corticosterone increase similarly in PACAP and wild-type mice. In addition, the restraint stress-induced c-Fos expression was significantly decreased in the paraventricular nucleus (PVN) and medial amygdala (MeA), but not the medial prefrontal cortex, in PACAP−/− mice. In the PVN of PACAP−/− mice, the stress-induced c-Fos expression was blunted in the CRF neurons. These results suggest that PACAP is critically involved in activation of the MeA and PVN CRF neurons to centrally regulate the HPA axis response to emotional stressors. |
| publisher |
Informa Healthcare |
| publishDate |
2011 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128825/ |
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1611463551567790080 |