STUDIES ON CARTILAGE : II. Electron Microscope Observations on Rabbit Ear Cartilage following the Administration of Papain

Electron microscope observations on rabbit ear cartilage following the administration of papain show that both the elastic component of the matrix and the amorphous material disappear leaving a matrix which consists of delicate fibrils which are presumed to be collagen. This unmasking of fibrils co...

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Main Authors:	Sheldon, Huntington, Robinson, Robert A.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1960
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224915/

id	pubmed-2224915
recordtype	oai_dc
spelling	pubmed-22249152008-05-01 STUDIES ON CARTILAGE : II. Electron Microscope Observations on Rabbit Ear Cartilage following the Administration of Papain Sheldon, Huntington Robinson, Robert A. Article Electron microscope observations on rabbit ear cartilage following the administration of papain show that both the elastic component of the matrix and the amorphous material disappear leaving a matrix which consists of delicate fibrils which are presumed to be collagen. This unmasking of fibrils coincides with the appearance of an abnormal component in the electrophoretic pattern of the rabbit's serum. The chondrocytes show vacuoles in their cytoplasm which appear at the same time that the cells appear crenated in the light microscope. A ruffly appearance of the cell surface membrane coincides with this vacuolization, and vacuoles often appear open and in continuity with the extracellular space. The resurgence of the rabbit ear is accompanied by a reconstitution of both the amorphous material and the elastic component of the matrix. During this period numerous dilated cisternae of the endoplasmic reticulum which contain a moderately dense material are present in the chondrocyte cytoplasm. We have been unable to demonstrate a direct relationship between the elastic component of the matrix and a particular component of the chondrocyte cytoplasm, but it is clear that changes occur in the cartilage cell cytoplasm during both the depletion and reconstitution of the matrix. Previous studies on the effect of papain on elastic tissue are noted and the possible relationships between changes in the cells and matrix of this elastic cartilage are discussed. The Rockefeller University Press 1960-09-01 /pmc/articles/PMC2224915/ /pubmed/19866569 Text en Copyright © Copyright 1961 by The Rockefeller Institute Press
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Sheldon, Huntington Robinson, Robert A.
spellingShingle	Sheldon, Huntington Robinson, Robert A. STUDIES ON CARTILAGE : II. Electron Microscope Observations on Rabbit Ear Cartilage following the Administration of Papain
author_facet	Sheldon, Huntington Robinson, Robert A.
author_sort	Sheldon, Huntington
title	STUDIES ON CARTILAGE : II. Electron Microscope Observations on Rabbit Ear Cartilage following the Administration of Papain
title_short	STUDIES ON CARTILAGE : II. Electron Microscope Observations on Rabbit Ear Cartilage following the Administration of Papain
title_full	STUDIES ON CARTILAGE : II. Electron Microscope Observations on Rabbit Ear Cartilage following the Administration of Papain
title_fullStr	STUDIES ON CARTILAGE : II. Electron Microscope Observations on Rabbit Ear Cartilage following the Administration of Papain
title_full_unstemmed	STUDIES ON CARTILAGE : II. Electron Microscope Observations on Rabbit Ear Cartilage following the Administration of Papain
title_sort	studies on cartilage : ii. electron microscope observations on rabbit ear cartilage following the administration of papain
description	Electron microscope observations on rabbit ear cartilage following the administration of papain show that both the elastic component of the matrix and the amorphous material disappear leaving a matrix which consists of delicate fibrils which are presumed to be collagen. This unmasking of fibrils coincides with the appearance of an abnormal component in the electrophoretic pattern of the rabbit's serum. The chondrocytes show vacuoles in their cytoplasm which appear at the same time that the cells appear crenated in the light microscope. A ruffly appearance of the cell surface membrane coincides with this vacuolization, and vacuoles often appear open and in continuity with the extracellular space. The resurgence of the rabbit ear is accompanied by a reconstitution of both the amorphous material and the elastic component of the matrix. During this period numerous dilated cisternae of the endoplasmic reticulum which contain a moderately dense material are present in the chondrocyte cytoplasm. We have been unable to demonstrate a direct relationship between the elastic component of the matrix and a particular component of the chondrocyte cytoplasm, but it is clear that changes occur in the cartilage cell cytoplasm during both the depletion and reconstitution of the matrix. Previous studies on the effect of papain on elastic tissue are noted and the possible relationships between changes in the cells and matrix of this elastic cartilage are discussed.
publisher	The Rockefeller University Press
publishDate	1960
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224915/
_version_	1611436483888021504

STUDIES ON CARTILAGE : II. Electron Microscope Observations on Rabbit Ear Cartilage following the Administration of Papain

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