An Alternative Open Reading Frame of the Human Macrophage Colony-Stimulating Factor Gene Is Independently Translated and Codes for an Antigenic Peptide of 14 Amino Acids Recognized by Tumor-Infiltrating Cd8 T Lymphocytes

We show that cytotoxic T lymphocytes (CTLs) infiltrating a kidney tumor recognize a peptide encoded by an alternative open reading frame (ORF) of the macrophage colony-stimulating factor (M-CSF) gene. Remarkably, this alternative ORF, which is translated in many tumors concurrently with the major OR...

Full description

Bibliographic Details
Main Authors:	Probst-Kepper, Michael, Stroobant, Vincent, Kridel, Robert, Gaugler, Béatrice, Landry, Claire, Brasseur, Francis, Cosyns, Jean-Pierre, Weynand, Birgit, Boon, Thierry, Van den Eynde, Benoit J.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 2001
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193327/

id	pubmed-2193327
recordtype	oai_dc
spelling	pubmed-21933272008-04-14 An Alternative Open Reading Frame of the Human Macrophage Colony-Stimulating Factor Gene Is Independently Translated and Codes for an Antigenic Peptide of 14 Amino Acids Recognized by Tumor-Infiltrating Cd8 T Lymphocytes Probst-Kepper, Michael Stroobant, Vincent Kridel, Robert Gaugler, Béatrice Landry, Claire Brasseur, Francis Cosyns, Jean-Pierre Weynand, Birgit Boon, Thierry Van den Eynde, Benoit J. Original Article We show that cytotoxic T lymphocytes (CTLs) infiltrating a kidney tumor recognize a peptide encoded by an alternative open reading frame (ORF) of the macrophage colony-stimulating factor (M-CSF) gene. Remarkably, this alternative ORF, which is translated in many tumors concurrently with the major ORF, is also translated in some tissues that do not produce M-CSF, such as liver and kidney. Such a dissociation of the translation of two overlapping ORFs from the same gene is unexpected. The antigenic peptide encoded by the alternative ORF is presented by human histocompatibility leukocyte antigen (HLA)-B*3501 and has a length of 14 residues. Peptide elution indicated that tumor cells naturally present this 14 mer, which is the longest peptide known to be recognized by CTLs. Binding studies of peptide analogues suggest that it binds by its two extremities and bulges out of the HLA groove to compensate for its length. The Rockefeller University Press 2001-05-21 /pmc/articles/PMC2193327/ /pubmed/11369790 Text en © 2001 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Probst-Kepper, Michael Stroobant, Vincent Kridel, Robert Gaugler, Béatrice Landry, Claire Brasseur, Francis Cosyns, Jean-Pierre Weynand, Birgit Boon, Thierry Van den Eynde, Benoit J.
spellingShingle	Probst-Kepper, Michael Stroobant, Vincent Kridel, Robert Gaugler, Béatrice Landry, Claire Brasseur, Francis Cosyns, Jean-Pierre Weynand, Birgit Boon, Thierry Van den Eynde, Benoit J. An Alternative Open Reading Frame of the Human Macrophage Colony-Stimulating Factor Gene Is Independently Translated and Codes for an Antigenic Peptide of 14 Amino Acids Recognized by Tumor-Infiltrating Cd8 T Lymphocytes
author_facet	Probst-Kepper, Michael Stroobant, Vincent Kridel, Robert Gaugler, Béatrice Landry, Claire Brasseur, Francis Cosyns, Jean-Pierre Weynand, Birgit Boon, Thierry Van den Eynde, Benoit J.
author_sort	Probst-Kepper, Michael
title	An Alternative Open Reading Frame of the Human Macrophage Colony-Stimulating Factor Gene Is Independently Translated and Codes for an Antigenic Peptide of 14 Amino Acids Recognized by Tumor-Infiltrating Cd8 T Lymphocytes
title_short	An Alternative Open Reading Frame of the Human Macrophage Colony-Stimulating Factor Gene Is Independently Translated and Codes for an Antigenic Peptide of 14 Amino Acids Recognized by Tumor-Infiltrating Cd8 T Lymphocytes
title_full	An Alternative Open Reading Frame of the Human Macrophage Colony-Stimulating Factor Gene Is Independently Translated and Codes for an Antigenic Peptide of 14 Amino Acids Recognized by Tumor-Infiltrating Cd8 T Lymphocytes
title_fullStr	An Alternative Open Reading Frame of the Human Macrophage Colony-Stimulating Factor Gene Is Independently Translated and Codes for an Antigenic Peptide of 14 Amino Acids Recognized by Tumor-Infiltrating Cd8 T Lymphocytes
title_full_unstemmed	An Alternative Open Reading Frame of the Human Macrophage Colony-Stimulating Factor Gene Is Independently Translated and Codes for an Antigenic Peptide of 14 Amino Acids Recognized by Tumor-Infiltrating Cd8 T Lymphocytes
title_sort	alternative open reading frame of the human macrophage colony-stimulating factor gene is independently translated and codes for an antigenic peptide of 14 amino acids recognized by tumor-infiltrating cd8 t lymphocytes
description	We show that cytotoxic T lymphocytes (CTLs) infiltrating a kidney tumor recognize a peptide encoded by an alternative open reading frame (ORF) of the macrophage colony-stimulating factor (M-CSF) gene. Remarkably, this alternative ORF, which is translated in many tumors concurrently with the major ORF, is also translated in some tissues that do not produce M-CSF, such as liver and kidney. Such a dissociation of the translation of two overlapping ORFs from the same gene is unexpected. The antigenic peptide encoded by the alternative ORF is presented by human histocompatibility leukocyte antigen (HLA)-B*3501 and has a length of 14 residues. Peptide elution indicated that tumor cells naturally present this 14 mer, which is the longest peptide known to be recognized by CTLs. Binding studies of peptide analogues suggest that it binds by its two extremities and bulges out of the HLA groove to compensate for its length.
publisher	The Rockefeller University Press
publishDate	2001
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193327/
_version_	1611431026167382016

An Alternative Open Reading Frame of the Human Macrophage Colony-Stimulating Factor Gene Is Independently Translated and Codes for an Antigenic Peptide of 14 Amino Acids Recognized by Tumor-Infiltrating Cd8 T Lymphocytes

Similar Items