The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation

It has been reported that nontransformed mammalian cells become arrested during G1 in the absence of centrioles (Hinchcliffe, E., F. Miller, M. Cham, A. Khodjakov, and G. Sluder. 2001. Science. 291:1547–1550). Here, we show that removal of resident centrioles (by laser ablation or needle microsurger...

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Main Authors:	La Terra, Sabrina, English, Christopher N., Hergert, Polla, McEwen, Bruce F., Sluder, Greenfield, Khodjakov, Alexey
Format:	Online
Language:	English
Published:	The Rockefeller University Press 2005
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171814/

id	pubmed-2171814
recordtype	oai_dc
spelling	pubmed-21718142008-03-05 The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation La Terra, Sabrina English, Christopher N. Hergert, Polla McEwen, Bruce F. Sluder, Greenfield Khodjakov, Alexey Research Articles It has been reported that nontransformed mammalian cells become arrested during G1 in the absence of centrioles (Hinchcliffe, E., F. Miller, M. Cham, A. Khodjakov, and G. Sluder. 2001. Science. 291:1547–1550). Here, we show that removal of resident centrioles (by laser ablation or needle microsurgery) does not impede cell cycle progression in HeLa cells. HeLa cells born without centrosomes, later, assemble a variable number of centrioles de novo. Centriole assembly begins with the formation of small centrin aggregates that appear during the S phase. These, initially amorphous “precentrioles” become morphologically recognizable centrioles before mitosis. De novo–assembled centrioles mature (i.e., gain abilities to organize microtubules and replicate) in the next cell cycle. This maturation is not simply a time-dependent phenomenon, because de novo–formed centrioles do not mature if they are assembled in S phase–arrested cells. By selectively ablating only one centriole at a time, we find that the presence of a single centriole inhibits the assembly of additional centrioles, indicating that centrioles have an activity that suppresses the de novo pathway. The Rockefeller University Press 2005-02-28 /pmc/articles/PMC2171814/ /pubmed/15738265 http://dx.doi.org/10.1083/jcb.200411126 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	La Terra, Sabrina English, Christopher N. Hergert, Polla McEwen, Bruce F. Sluder, Greenfield Khodjakov, Alexey
spellingShingle	La Terra, Sabrina English, Christopher N. Hergert, Polla McEwen, Bruce F. Sluder, Greenfield Khodjakov, Alexey The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation
author_facet	La Terra, Sabrina English, Christopher N. Hergert, Polla McEwen, Bruce F. Sluder, Greenfield Khodjakov, Alexey
author_sort	La Terra, Sabrina
title	The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation
title_short	The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation
title_full	The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation
title_fullStr	The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation
title_full_unstemmed	The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation
title_sort	de novo centriole assembly pathway in hela cells: cell cycle progression and centriole assembly/maturation
description	It has been reported that nontransformed mammalian cells become arrested during G1 in the absence of centrioles (Hinchcliffe, E., F. Miller, M. Cham, A. Khodjakov, and G. Sluder. 2001. Science. 291:1547–1550). Here, we show that removal of resident centrioles (by laser ablation or needle microsurgery) does not impede cell cycle progression in HeLa cells. HeLa cells born without centrosomes, later, assemble a variable number of centrioles de novo. Centriole assembly begins with the formation of small centrin aggregates that appear during the S phase. These, initially amorphous “precentrioles” become morphologically recognizable centrioles before mitosis. De novo–assembled centrioles mature (i.e., gain abilities to organize microtubules and replicate) in the next cell cycle. This maturation is not simply a time-dependent phenomenon, because de novo–formed centrioles do not mature if they are assembled in S phase–arrested cells. By selectively ablating only one centriole at a time, we find that the presence of a single centriole inhibits the assembly of additional centrioles, indicating that centrioles have an activity that suppresses the de novo pathway.
publisher	The Rockefeller University Press
publishDate	2005
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171814/
_version_	1611424719536390144

The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation

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