Isolation and characterization of sea urchin egg cortical granules
A method has been developed to isolate cortical granules (CG) free in suspension. It involves the mechanical disruption of the CG from CG lawns (CGL; Dev. Biol. 43:62-74, 1975) and concentration of the CG by low speed centrifugation. The isolated CG are intact and are a relatively pure population as...
Format: | Online |
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Language: | English |
Published: |
The Rockefeller University Press
1982
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112902/ |