Isolation and characterization of the vitelline layer of sea urchin eggs
The vitelline layers (VLs) of unfertilized sea urchin eggs were isolated by homogenization in a hypotonic medium containing Triton X- 100 and EDTA. The surface topography of the VL is not changed by isolation. The thickness of the isolated VLs (300-400 A) is greater than that reported for VLs on int...
| Format: | Online |
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| Language: | English |
| Published: |
The Rockefeller University Press
1977
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109943/ |