Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting

The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres where it provides the cohesive counterforce to bipolar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences transie...

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Main Authors:	Ocampo-Hafalla, Maria T., Katou, Yuki, Shirahige, Katsuhiko, Uhlmann, Frank
Format:	Online
Language:	English
Published:	Springer-Verlag 2007
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075529/

id	pubmed-2075529
recordtype	oai_dc
spelling	pubmed-20755292007-11-15 Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting Ocampo-Hafalla, Maria T. Katou, Yuki Shirahige, Katsuhiko Uhlmann, Frank Research Article The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres where it provides the cohesive counterforce to bipolar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences transiently split in pre-anaphase cells, in some organisms up to several micrometers. This ‘centromere breathing’ presents a paradox, how sister sequences separate where cohesin is most enriched. We now show that in the budding yeast Saccharomyces cerevisiae, cohesin binding diminishes over centromeric sequences that split during breathing. We see no evidence for cohesin translocation to surrounding sequences, suggesting that cohesin is removed from centromeres during breathing. Two pools of cohesin can be distinguished. Cohesin loaded before DNA replication, which has established sister chromatid cohesion, disappears during breathing. In contrast, cohesin loaded after DNA replication is partly retained. As sister centromeres re-associate after transient separation, cohesin is reloaded in a manner independent of the canonical cohesin loader Scc2/Scc4. Efficient centromere re-association requires the cohesion establishment factor Eco1, suggesting that re-establishment of sister chromatid cohesion contributes to the dynamic behaviour of centromeres in mitosis. These findings provide new insights into cohesin behaviour at centromeres. Springer-Verlag 2007-09-01 2007-12 /pmc/articles/PMC2075529/ /pubmed/17763979 http://dx.doi.org/10.1007/s00412-007-0118-4 Text en © Springer-Verlag 2007
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ocampo-Hafalla, Maria T. Katou, Yuki Shirahige, Katsuhiko Uhlmann, Frank
spellingShingle	Ocampo-Hafalla, Maria T. Katou, Yuki Shirahige, Katsuhiko Uhlmann, Frank Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
author_facet	Ocampo-Hafalla, Maria T. Katou, Yuki Shirahige, Katsuhiko Uhlmann, Frank
author_sort	Ocampo-Hafalla, Maria T.
title	Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
title_short	Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
title_full	Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
title_fullStr	Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
title_full_unstemmed	Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
title_sort	displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
description	The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres where it provides the cohesive counterforce to bipolar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences transiently split in pre-anaphase cells, in some organisms up to several micrometers. This ‘centromere breathing’ presents a paradox, how sister sequences separate where cohesin is most enriched. We now show that in the budding yeast Saccharomyces cerevisiae, cohesin binding diminishes over centromeric sequences that split during breathing. We see no evidence for cohesin translocation to surrounding sequences, suggesting that cohesin is removed from centromeres during breathing. Two pools of cohesin can be distinguished. Cohesin loaded before DNA replication, which has established sister chromatid cohesion, disappears during breathing. In contrast, cohesin loaded after DNA replication is partly retained. As sister centromeres re-associate after transient separation, cohesin is reloaded in a manner independent of the canonical cohesin loader Scc2/Scc4. Efficient centromere re-association requires the cohesion establishment factor Eco1, suggesting that re-establishment of sister chromatid cohesion contributes to the dynamic behaviour of centromeres in mitosis. These findings provide new insights into cohesin behaviour at centromeres.
publisher	Springer-Verlag
publishDate	2007
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075529/
_version_	1611407975259308032

Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting

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