The transcriptional cycle of HIV-1 in real-time and live cells

RNA polymerase II (RNAPII) is a fundamental enzyme, but few studies have analyzed its activity in living cells. Using human immunodeficiency virus (HIV) type 1 reporters, we study real-time messenger RNA (mRNA) biogenesis by photobleaching nascent RNAs and RNAPII at specific transcription sites. Thr...

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Bibliographic Details
Main Authors: Boireau, Stéphanie, Maiuri, Paolo, Basyuk, Eugenia, de la Mata, Manuel, Knezevich, Anna, Pradet-Balade, Bérangère, Bäcker, Volker, Kornblihtt, Alberto, Marcello, Alessandro, Bertrand, Edouard
Format: Online
Language:English
Published: The Rockefeller University Press 2007
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064765/