Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed

Anumber of mammalian genes are expressed from only one of the two homologous chromosomes, selected at random in each cell. These include genes subject to X-inactivation, olfactory receptor (OR) genes, and several classes of immune system genes. The means by which monoallelic expression is establishe...

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Main Authors:	Alexander, Mary Kate, Mlynarczyk-Evans, Susanna, Royce-Tolland, Morgan, Plocik, Alex, Kalantry, Sundeep, Magnuson, Terry, Panning, Barbara
Format:	Online
Language:	English
Published:	The Rockefeller University Press 2007
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064763/

id	pubmed-2064763
recordtype	oai_dc
spelling	pubmed-20647632008-04-22 Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed Alexander, Mary Kate Mlynarczyk-Evans, Susanna Royce-Tolland, Morgan Plocik, Alex Kalantry, Sundeep Magnuson, Terry Panning, Barbara Research Articles Anumber of mammalian genes are expressed from only one of the two homologous chromosomes, selected at random in each cell. These include genes subject to X-inactivation, olfactory receptor (OR) genes, and several classes of immune system genes. The means by which monoallelic expression is established are only beginning to be understood. Using a cytological assay, we show that the two homologous alleles of autosomal random monoallelic loci differ from each other in embryonic stem (ES) cells, before establishment of monoallelic expression. The Polycomb Group gene Eed is required to establish this distinctive behavior. In addition, we found that when Eed mutant ES cells are differentiated, they fail to establish asynchronous replication timing at OR loci. These results suggest a common mechanism for random monoallelic expression on autosomes and the X chromosome, and implicate Eed in establishing differences between homologous OR loci before and after differentiation. The Rockefeller University Press 2007-10-22 /pmc/articles/PMC2064763/ /pubmed/17954609 http://dx.doi.org/10.1083/jcb.200706053 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Alexander, Mary Kate Mlynarczyk-Evans, Susanna Royce-Tolland, Morgan Plocik, Alex Kalantry, Sundeep Magnuson, Terry Panning, Barbara
spellingShingle	Alexander, Mary Kate Mlynarczyk-Evans, Susanna Royce-Tolland, Morgan Plocik, Alex Kalantry, Sundeep Magnuson, Terry Panning, Barbara Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed
author_facet	Alexander, Mary Kate Mlynarczyk-Evans, Susanna Royce-Tolland, Morgan Plocik, Alex Kalantry, Sundeep Magnuson, Terry Panning, Barbara
author_sort	Alexander, Mary Kate
title	Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed
title_short	Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed
title_full	Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed
title_fullStr	Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed
title_full_unstemmed	Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed
title_sort	differences between homologous alleles of olfactory receptor genes require the polycomb group protein eed
description	Anumber of mammalian genes are expressed from only one of the two homologous chromosomes, selected at random in each cell. These include genes subject to X-inactivation, olfactory receptor (OR) genes, and several classes of immune system genes. The means by which monoallelic expression is established are only beginning to be understood. Using a cytological assay, we show that the two homologous alleles of autosomal random monoallelic loci differ from each other in embryonic stem (ES) cells, before establishment of monoallelic expression. The Polycomb Group gene Eed is required to establish this distinctive behavior. In addition, we found that when Eed mutant ES cells are differentiated, they fail to establish asynchronous replication timing at OR loci. These results suggest a common mechanism for random monoallelic expression on autosomes and the X chromosome, and implicate Eed in establishing differences between homologous OR loci before and after differentiation.
publisher	The Rockefeller University Press
publishDate	2007
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064763/
_version_	1611406825743187968

Differences between homologous alleles of olfactory receptor genes require the Polycomb Group protein Eed

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