DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes

Infection of T cells by HIV-1 can occur through binding of virus to dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) on dendritic cells and transfer of virus to CD4+ T cells. Here we show that a subset of B cells in the blood and tonsils of normal donors expressed DC-SIGN, and that...

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Main Authors:	Rappocciolo, Giovanna, Piazza, Paolo, Fuller, Craig L, Reinhart, Todd A, Watkins, Simon C, Rowe, David T, Jais, Mariel, Gupta, Phalguni, Rinaldo, Charles R
Format:	Online
Language:	English
Published:	Public Library of Science 2006
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1500807/

id	pubmed-1500807
recordtype	oai_dc
spelling	pubmed-15008072006-07-14 DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes Rappocciolo, Giovanna Piazza, Paolo Fuller, Craig L Reinhart, Todd A Watkins, Simon C Rowe, David T Jais, Mariel Gupta, Phalguni Rinaldo, Charles R Research Article Infection of T cells by HIV-1 can occur through binding of virus to dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) on dendritic cells and transfer of virus to CD4+ T cells. Here we show that a subset of B cells in the blood and tonsils of normal donors expressed DC-SIGN, and that this increased after stimulation in vitro with interleukin 4 and CD40 ligand, with enhanced expression of activation and co-stimulatory molecules CD23, CD58, CD80, and CD86, and CD22. The activated B cells captured and internalized X4 and R5 tropic strains of HIV-1, and mediated trans infection of T cells. Pretreatment of the B cells with anti–DC-SIGN monoclonal antibody blocked trans infection of T cells by both strains of HIV-1. These results indicate that DC-SIGN serves as a portal on B cells for HIV-1 infection of T cells in trans. Transmission of HIV-1 from B cells to T cells through this DC-SIGN pathway could be important in the pathogenesis of HIV-1 infection. Public Library of Science 2006-07 2006-07-14 /pmc/articles/PMC1500807/ /pubmed/16839201 http://dx.doi.org/10.1371/journal.ppat.0020070 Text en © 2006 Rappocciolo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Rappocciolo, Giovanna Piazza, Paolo Fuller, Craig L Reinhart, Todd A Watkins, Simon C Rowe, David T Jais, Mariel Gupta, Phalguni Rinaldo, Charles R
spellingShingle	Rappocciolo, Giovanna Piazza, Paolo Fuller, Craig L Reinhart, Todd A Watkins, Simon C Rowe, David T Jais, Mariel Gupta, Phalguni Rinaldo, Charles R DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes
author_facet	Rappocciolo, Giovanna Piazza, Paolo Fuller, Craig L Reinhart, Todd A Watkins, Simon C Rowe, David T Jais, Mariel Gupta, Phalguni Rinaldo, Charles R
author_sort	Rappocciolo, Giovanna
title	DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes
title_short	DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes
title_full	DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes
title_fullStr	DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes
title_full_unstemmed	DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes
title_sort	dc-sign on b lymphocytes is required for transmission of hiv-1 to t lymphocytes
description	Infection of T cells by HIV-1 can occur through binding of virus to dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) on dendritic cells and transfer of virus to CD4+ T cells. Here we show that a subset of B cells in the blood and tonsils of normal donors expressed DC-SIGN, and that this increased after stimulation in vitro with interleukin 4 and CD40 ligand, with enhanced expression of activation and co-stimulatory molecules CD23, CD58, CD80, and CD86, and CD22. The activated B cells captured and internalized X4 and R5 tropic strains of HIV-1, and mediated trans infection of T cells. Pretreatment of the B cells with anti–DC-SIGN monoclonal antibody blocked trans infection of T cells by both strains of HIV-1. These results indicate that DC-SIGN serves as a portal on B cells for HIV-1 infection of T cells in trans. Transmission of HIV-1 from B cells to T cells through this DC-SIGN pathway could be important in the pathogenesis of HIV-1 infection.
publisher	Public Library of Science
publishDate	2006
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1500807/
_version_	1611384495411298304

DC-SIGN on B Lymphocytes Is Required For Transmission of HIV-1 to T Lymphocytes

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