A single catalytic domain of the junction-resolving enzyme T7 endonuclease I is a non-specific nicking endonuclease
A stable heterodimeric protein containing a single correctly folded catalytic domain (SCD) of T7 endonuclease I was produced by means of a trans-splicing intein system. As predicted by a model presented earlier, purified SCD protein acts a non-specific nicking endonuclease on normal linear DNA. The...
| Main Authors: | , |
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| Format: | Online |
| Language: | English |
| Published: |
Oxford University Press
2005
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1277808/ |