Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes
We describe the construction and application of elements for random insertion of promoter containing DNA into the genome of Bacillus subtilis. The outward-facing promoter of these integrative elements termed InsTetG+ is inducible by tetracycline so that conditional mutants are generated. We construc...
Main Authors: | Bertram, Ralph, Köstner, Martin, Müller, Judith, Ramos, José Vazquez, Hillen, Wolfgang |
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Format: | Online |
Language: | English |
Published: |
Oxford University Press
2005
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1253839/ |
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