Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes
We describe the construction and application of elements for random insertion of promoter containing DNA into the genome of Bacillus subtilis. The outward-facing promoter of these integrative elements termed InsTetG+ is inducible by tetracycline so that conditional mutants are generated. We construc...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Oxford University Press
2005
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1253839/ |