Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats

Secondary structures of the G-rich strand of human telomere DNA fragments G3(TTAG3)n, n = 1–16, have been studied by means of circular dichroism spectroscopy and PAGE, in solutions of physiological potassium cation concentrations. It has been found that folding of these fragments into tetraplexes as...

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Main Authors:	Vorlíčková, Michaela, Chládková, Jana, Kejnovská, Iva, Fialová, Markéta, Kypr, Jaroslav
Format:	Online
Language:	English
Published:	Oxford University Press 2005
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1253834/

id	pubmed-1253834
recordtype	oai_dc
spelling	pubmed-12538342005-10-14 Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats Vorlíčková, Michaela Chládková, Jana Kejnovská, Iva Fialová, Markéta Kypr, Jaroslav Article Secondary structures of the G-rich strand of human telomere DNA fragments G3(TTAG3)n, n = 1–16, have been studied by means of circular dichroism spectroscopy and PAGE, in solutions of physiological potassium cation concentrations. It has been found that folding of these fragments into tetraplexes as well as tetraplex thermostabilities and enthalpy values depend on the number of TTAG3 repeats. The suggested topologies include, e.g. antiparallel and parallel bimolecular tetraplexes, an intramolecular antiparallel tetraplex, a tetraplex consisting of three parallel chains and one antiparallel chain, a poorly stable parallel intramolecular tetraplex, and both parallel and antiparallel tetramolecular tetraplexes. G3(TTAG3)3 folds into a single, stable and very compact intramolecular antiparallel tetraplex. With an increasing repeat number, the fragment tetraplexes surprisingly are ever less thermostable and their migration and enthalpy decrease indicate increasing irregularities or domain splitting in their arrangements. Reduced stability and different topology of lengthy telomeric tails could contribute to the stepwise telomere shortening process. Oxford University Press 2005 2005-10-12 /pmc/articles/PMC1253834/ /pubmed/16221978 http://dx.doi.org/10.1093/nar/gki898 Text en © The Author 2005. Published by Oxford University Press. All rights reserved
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Vorlíčková, Michaela Chládková, Jana Kejnovská, Iva Fialová, Markéta Kypr, Jaroslav
spellingShingle	Vorlíčková, Michaela Chládková, Jana Kejnovská, Iva Fialová, Markéta Kypr, Jaroslav Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats
author_facet	Vorlíčková, Michaela Chládková, Jana Kejnovská, Iva Fialová, Markéta Kypr, Jaroslav
author_sort	Vorlíčková, Michaela
title	Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats
title_short	Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats
title_full	Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats
title_fullStr	Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats
title_full_unstemmed	Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats
title_sort	guanine tetraplex topology of human telomere dna is governed by the number of (ttaggg) repeats
description	Secondary structures of the G-rich strand of human telomere DNA fragments G3(TTAG3)n, n = 1–16, have been studied by means of circular dichroism spectroscopy and PAGE, in solutions of physiological potassium cation concentrations. It has been found that folding of these fragments into tetraplexes as well as tetraplex thermostabilities and enthalpy values depend on the number of TTAG3 repeats. The suggested topologies include, e.g. antiparallel and parallel bimolecular tetraplexes, an intramolecular antiparallel tetraplex, a tetraplex consisting of three parallel chains and one antiparallel chain, a poorly stable parallel intramolecular tetraplex, and both parallel and antiparallel tetramolecular tetraplexes. G3(TTAG3)3 folds into a single, stable and very compact intramolecular antiparallel tetraplex. With an increasing repeat number, the fragment tetraplexes surprisingly are ever less thermostable and their migration and enthalpy decrease indicate increasing irregularities or domain splitting in their arrangements. Reduced stability and different topology of lengthy telomeric tails could contribute to the stepwise telomere shortening process.
publisher	Oxford University Press
publishDate	2005
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1253834/
_version_	1611378580343750656

Guanine tetraplex topology of human telomere DNA is governed by the number of (TTAGGG) repeats

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