Torque-limited RecA polymerization on dsDNA
The assembly of RecA onto a torsionally constrained double-stranded DNA molecule was followed in real time using magnetic tweezers. Formation of a RecA–DNA filament on the DNA tether was stalled owing to different physical processes depending on the applied stretching force. For forces up to 3.6 pN,...
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| Format: | Online |
| Language: | English |
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Oxford University Press
2005
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1075924/ |
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pubmed-1075924 |
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oai_dc |
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pubmed-10759242005-04-11 Torque-limited RecA polymerization on dsDNA van der Heijden, Thijn van Noort, John van Leest, Hendrikje Kanaar, Roland Wyman, Claire Dekker, Nynke Dekker, Cees Article The assembly of RecA onto a torsionally constrained double-stranded DNA molecule was followed in real time using magnetic tweezers. Formation of a RecA–DNA filament on the DNA tether was stalled owing to different physical processes depending on the applied stretching force. For forces up to 3.6 pN, the reaction stalled owing to the formation of positive plectonemes in the remaining DNA molecule. Release of these plectonemes by rotation of the magnets led to full coverage of the DNA molecule by RecA. At stretching forces larger than 3.6 pN, the twist induced during filament formation caused the reaction to stall before positive supercoils were generated. We deduce a maximum built-up torsion of 10.1 ± 0.7 kbT. In vivo this built-up torsion may be used to favor regression of a stalled replication fork or to free the chromosomal DNA in E.coli from its condensing proteins. Oxford University Press 2005 2005-04-11 /pmc/articles/PMC1075924/ /pubmed/15824062 http://dx.doi.org/10.1093/nar/gki512 Text en © The Author 2005. Published by Oxford University Press. All rights reserved |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
van der Heijden, Thijn van Noort, John van Leest, Hendrikje Kanaar, Roland Wyman, Claire Dekker, Nynke Dekker, Cees |
| spellingShingle |
van der Heijden, Thijn van Noort, John van Leest, Hendrikje Kanaar, Roland Wyman, Claire Dekker, Nynke Dekker, Cees Torque-limited RecA polymerization on dsDNA |
| author_facet |
van der Heijden, Thijn van Noort, John van Leest, Hendrikje Kanaar, Roland Wyman, Claire Dekker, Nynke Dekker, Cees |
| author_sort |
van der Heijden, Thijn |
| title |
Torque-limited RecA polymerization on dsDNA |
| title_short |
Torque-limited RecA polymerization on dsDNA |
| title_full |
Torque-limited RecA polymerization on dsDNA |
| title_fullStr |
Torque-limited RecA polymerization on dsDNA |
| title_full_unstemmed |
Torque-limited RecA polymerization on dsDNA |
| title_sort |
torque-limited reca polymerization on dsdna |
| description |
The assembly of RecA onto a torsionally constrained double-stranded DNA molecule was followed in real time using magnetic tweezers. Formation of a RecA–DNA filament on the DNA tether was stalled owing to different physical processes depending on the applied stretching force. For forces up to 3.6 pN, the reaction stalled owing to the formation of positive plectonemes in the remaining DNA molecule. Release of these plectonemes by rotation of the magnets led to full coverage of the DNA molecule by RecA. At stretching forces larger than 3.6 pN, the twist induced during filament formation caused the reaction to stall before positive supercoils were generated. We deduce a maximum built-up torsion of 10.1 ± 0.7 kbT. In vivo this built-up torsion may be used to favor regression of a stalled replication fork or to free the chromosomal DNA in E.coli from its condensing proteins. |
| publisher |
Oxford University Press |
| publishDate |
2005 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1075924/ |
| _version_ |
1611373238169894912 |