Torque-limited RecA polymerization on dsDNA

The assembly of RecA onto a torsionally constrained double-stranded DNA molecule was followed in real time using magnetic tweezers. Formation of a RecA–DNA filament on the DNA tether was stalled owing to different physical processes depending on the applied stretching force. For forces up to 3.6 pN,...

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Bibliographic Details
Main Authors: van der Heijden, Thijn, van Noort, John, van Leest, Hendrikje, Kanaar, Roland, Wyman, Claire, Dekker, Nynke, Dekker, Cees
Format: Online
Language:English
Published: Oxford University Press 2005
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1075924/