An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi
RNA interference (RNAi) mediates gene silencing in many eukaryotes and has been widely used to investigate gene functions. A common method to induce sustained RNAi is introducing plasmids that synthesize short hairpin RNAs (shRNAs) using Pol III promoters. While these promoters synthesize shRNAs and...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
Oxford University Press
2005
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1074311/ |