Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk

A survey of retail purchased semi-skimmed pasteurised milk (n = 368) for Mycobacterium avium subspecies paratuberculosis (MAP) was conducted between May 2014 and June 2015 across the midlands of England using the Phage-PCR assay. Overall, 10.3% of the total samples collected contained viable MAP cel...

Full description

Bibliographic Details
Main Authors:	Gerrard, Zara E., Swift, Benjamin M.C., Botsaris, George, Davidson, Ross S., Hutchings, Michael R., Huxley, Jonathon N., Rees, Catherine E.D.
Format:	Article
Language:	English
Published:	Elsevier 2018
Online Access:	http://eprints.nottingham.ac.uk/52573/ http://eprints.nottingham.ac.uk/52573/ http://eprints.nottingham.ac.uk/52573/ http://eprints.nottingham.ac.uk/52573/1/Gerrard%20et%20al.%20Food%20Microbiology%20Accepted%20manuscript%202018%20Survival%20of%20MAP%20in%20retail%20pasteurised%20milk_.pdf

id	nottingham-52573
recordtype	eprints
spelling	nottingham-525732018-06-25T08:00:12Z http://eprints.nottingham.ac.uk/52573/ Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk Gerrard, Zara E. Swift, Benjamin M.C. Botsaris, George Davidson, Ross S. Hutchings, Michael R. Huxley, Jonathon N. Rees, Catherine E.D. A survey of retail purchased semi-skimmed pasteurised milk (n = 368) for Mycobacterium avium subspecies paratuberculosis (MAP) was conducted between May 2014 and June 2015 across the midlands of England using the Phage-PCR assay. Overall, 10.3% of the total samples collected contained viable MAP cells, confirming that pasteurisation is not capable of fully eliminating human exposure to viable MAP through milk. Comparison of the results gained using the Phage-PCR assay with the results of surveys using either culture or direct PCR suggest that the phage-PCR assay is able to detect lower numbers of cells, resulting in an increase in the number of MAP-positive samples detected. Comparison of viable count and levels of MAP detected in bulk milk samples suggest that MAP is not primarily introduced into the milk by faecal contamination but rather are shed directly into the milk within the udder. In addition results detected an asymmetric distribution of MAP exists in the milk matrix prior to somatic cell lysis, indicating that the bacterial cells in naturally contaminated milk are clustered together and may primarily be located within somatic cells. These latter two results lead to the hypothesis that intracellular MAP within the somatic cells may be protected against heat inactivation during pasteurisation, accounting for the presence of low levels of MAP detected in retail milk. Elsevier 2018-09-30 Article PeerReviewed application/pdf en cc_by_nc_nd http://eprints.nottingham.ac.uk/52573/1/Gerrard%20et%20al.%20Food%20Microbiology%20Accepted%20manuscript%202018%20Survival%20of%20MAP%20in%20retail%20pasteurised%20milk_.pdf Gerrard, Zara E. and Swift, Benjamin M.C. and Botsaris, George and Davidson, Ross S. and Hutchings, Michael R. and Huxley, Jonathon N. and Rees, Catherine E.D. (2018) Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk. Food Microbiology, 74 . pp. 57-63. ISSN 1095-9998 http://dx.doi.org/10.1016/j.fm.2018.03.004 doi:10.1016/j.fm.2018.03.004 doi:10.1016/j.fm.2018.03.004
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	A survey of retail purchased semi-skimmed pasteurised milk (n = 368) for Mycobacterium avium subspecies paratuberculosis (MAP) was conducted between May 2014 and June 2015 across the midlands of England using the Phage-PCR assay. Overall, 10.3% of the total samples collected contained viable MAP cells, confirming that pasteurisation is not capable of fully eliminating human exposure to viable MAP through milk. Comparison of the results gained using the Phage-PCR assay with the results of surveys using either culture or direct PCR suggest that the phage-PCR assay is able to detect lower numbers of cells, resulting in an increase in the number of MAP-positive samples detected. Comparison of viable count and levels of MAP detected in bulk milk samples suggest that MAP is not primarily introduced into the milk by faecal contamination but rather are shed directly into the milk within the udder. In addition results detected an asymmetric distribution of MAP exists in the milk matrix prior to somatic cell lysis, indicating that the bacterial cells in naturally contaminated milk are clustered together and may primarily be located within somatic cells. These latter two results lead to the hypothesis that intracellular MAP within the somatic cells may be protected against heat inactivation during pasteurisation, accounting for the presence of low levels of MAP detected in retail milk.
format	Article
author	Gerrard, Zara E. Swift, Benjamin M.C. Botsaris, George Davidson, Ross S. Hutchings, Michael R. Huxley, Jonathon N. Rees, Catherine E.D.
spellingShingle	Gerrard, Zara E. Swift, Benjamin M.C. Botsaris, George Davidson, Ross S. Hutchings, Michael R. Huxley, Jonathon N. Rees, Catherine E.D. Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk
author_facet	Gerrard, Zara E. Swift, Benjamin M.C. Botsaris, George Davidson, Ross S. Hutchings, Michael R. Huxley, Jonathon N. Rees, Catherine E.D.
author_sort	Gerrard, Zara E.
title	Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk
title_short	Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk
title_full	Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk
title_fullStr	Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk
title_full_unstemmed	Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk
title_sort	survival of mycobacterium avium subspecies paratuberculosis in retail pasteurised milk
publisher	Elsevier
publishDate	2018
url	http://eprints.nottingham.ac.uk/52573/ http://eprints.nottingham.ac.uk/52573/ http://eprints.nottingham.ac.uk/52573/ http://eprints.nottingham.ac.uk/52573/1/Gerrard%20et%20al.%20Food%20Microbiology%20Accepted%20manuscript%202018%20Survival%20of%20MAP%20in%20retail%20pasteurised%20milk_.pdf
first_indexed	2018-09-06T14:28:01Z
last_indexed	2018-09-06T14:28:01Z
_version_	1610868674499969024

Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk

Similar Items