Hyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stress

Abstract Background Currently, two pathogenic pathways describe the role of obesity in osteoarthritis (OA); one through biomechanical stress, and the other by the contribution of systemic inflammation. The aim of this study was to evaluate the effect of free fatty acids (FFA) in human chondrocytes (...

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Main Authors:	Daniel Medina-Luna, Mónica Guadalupe Santamaría-Olmedo, Yessica Zamudio-Cuevas, Karina Martínez-Flores, Javier Fernández-Torres, Gabriela Angélica Martínez-Nava, Denise Clavijo-Cornejo, Cristina Hernández-Díaz, Anell Olivos-Meza, Luis Enrique Gomez-Quiroz, María Concepción Gutiérrez-Ruiz, Carlos Pineda, Francisco Blanco, Anthony M. Reginato, Alberto López-Reyes
Format:	Article
Language:	English
Published:	BioMed Central 2017-06-01
Series:	Lipids in Health and Disease
Subjects:	Chondrocytes Free fatty acids Inflammation Oxidative stress
Online Access:	http://link.springer.com/article/10.1186/s12944-017-0510-x

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spelling	doaj-art-808d32b00c3643f6b01a9b02b728329d2018-08-20T18:20:08ZengBioMed CentralLipids in Health and Disease1476-511X2017-06-011611810.1186/s12944-017-0510-xHyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stressDaniel Medina-Luna0Mónica Guadalupe Santamaría-Olmedo1Yessica Zamudio-Cuevas2Karina Martínez-Flores3Javier Fernández-Torres4Gabriela Angélica Martínez-Nava5Denise Clavijo-Cornejo6Cristina Hernández-Díaz7Anell Olivos-Meza8Luis Enrique Gomez-Quiroz9María Concepción Gutiérrez-Ruiz10Carlos Pineda11Francisco Blanco12Anthony M. Reginato13Alberto López-Reyes14Synovial Fluid Laboratory, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra”Synovial Fluid Laboratory, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra”Synovial Fluid Laboratory, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra”Synovial Fluid Laboratory, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra”Synovial Fluid Laboratory, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra”Synovial Fluid Laboratory, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra”Synovial Fluid Laboratory, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra”Musculoeskeletal and Articular Ultrasound LaboratoryArthroscopy Service; Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra Ibarra”, Secretaría de SaludDepartamento de Ciencias de la Salud, Universidad Autónoma Metropolitana IztapalapaDepartamento de Ciencias de la Salud, Universidad Autónoma Metropolitana IztapalapaMusculoeskeletal and Articular Ultrasound LaboratoryRheumatology Division, ProteoRed/ISC III Proteomics Group, INBICDivision of Rheumatology, Warren Alpert School of Medicine at Brown UniversitySynovial Fluid Laboratory, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra”Abstract Background Currently, two pathogenic pathways describe the role of obesity in osteoarthritis (OA); one through biomechanical stress, and the other by the contribution of systemic inflammation. The aim of this study was to evaluate the effect of free fatty acids (FFA) in human chondrocytes (HC) expression of proinflammatory factors and reactive oxygen species (ROS). Methods HC were exposed to two different concentrations of FFA in order to evaluate the secretion of adipokines through cytokines immunoassays panel, quantify the protein secretion of FFA-treated chondrocytes, and fluorescent cytometry assays were performed to evaluate the reactive oxygen species (ROS) production. Results HC injury was observed at 48 h of treatment with FFA. In the FFA-treated HC the production of reactive oxygen species such as superoxide radical, hydrogen peroxide, and the reactive nitrogen species increased significantly in a at the two-dose tested (250 and 500 μM). In addition, we found an increase in the cytokine secretion of IL-6 and chemokine IL-8 in FFA-treated HC in comparison to the untreated HC. Conclusion In our in vitro model of HC, a hyperlipidemia microenvironment induces an oxidative stress state that enhances the inflammatory process mediated by adipokines secretion in HC.http://link.springer.com/article/10.1186/s12944-017-0510-xChondrocytesFree fatty acidsInflammationOxidative stress
institution	Open Data Bank
collection	Open Access Journals
building	Directory of Open Access Journals
language	English
format	Article
author	Daniel Medina-Luna Mónica Guadalupe Santamaría-Olmedo Yessica Zamudio-Cuevas Karina Martínez-Flores Javier Fernández-Torres Gabriela Angélica Martínez-Nava Denise Clavijo-Cornejo Cristina Hernández-Díaz Anell Olivos-Meza Luis Enrique Gomez-Quiroz María Concepción Gutiérrez-Ruiz Carlos Pineda Francisco Blanco Anthony M. Reginato Alberto López-Reyes
spellingShingle	Daniel Medina-Luna Mónica Guadalupe Santamaría-Olmedo Yessica Zamudio-Cuevas Karina Martínez-Flores Javier Fernández-Torres Gabriela Angélica Martínez-Nava Denise Clavijo-Cornejo Cristina Hernández-Díaz Anell Olivos-Meza Luis Enrique Gomez-Quiroz María Concepción Gutiérrez-Ruiz Carlos Pineda Francisco Blanco Anthony M. Reginato Alberto López-Reyes Hyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stress Lipids in Health and Disease Chondrocytes Free fatty acids Inflammation Oxidative stress
author_facet	Daniel Medina-Luna Mónica Guadalupe Santamaría-Olmedo Yessica Zamudio-Cuevas Karina Martínez-Flores Javier Fernández-Torres Gabriela Angélica Martínez-Nava Denise Clavijo-Cornejo Cristina Hernández-Díaz Anell Olivos-Meza Luis Enrique Gomez-Quiroz María Concepción Gutiérrez-Ruiz Carlos Pineda Francisco Blanco Anthony M. Reginato Alberto López-Reyes
author_sort	Daniel Medina-Luna
title	Hyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stress
title_short	Hyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stress
title_full	Hyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stress
title_fullStr	Hyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stress
title_full_unstemmed	Hyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stress
title_sort	hyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stress
publisher	BioMed Central
series	Lipids in Health and Disease
issn	1476-511X
publishDate	2017-06-01
description	Abstract Background Currently, two pathogenic pathways describe the role of obesity in osteoarthritis (OA); one through biomechanical stress, and the other by the contribution of systemic inflammation. The aim of this study was to evaluate the effect of free fatty acids (FFA) in human chondrocytes (HC) expression of proinflammatory factors and reactive oxygen species (ROS). Methods HC were exposed to two different concentrations of FFA in order to evaluate the secretion of adipokines through cytokines immunoassays panel, quantify the protein secretion of FFA-treated chondrocytes, and fluorescent cytometry assays were performed to evaluate the reactive oxygen species (ROS) production. Results HC injury was observed at 48 h of treatment with FFA. In the FFA-treated HC the production of reactive oxygen species such as superoxide radical, hydrogen peroxide, and the reactive nitrogen species increased significantly in a at the two-dose tested (250 and 500 μM). In addition, we found an increase in the cytokine secretion of IL-6 and chemokine IL-8 in FFA-treated HC in comparison to the untreated HC. Conclusion In our in vitro model of HC, a hyperlipidemia microenvironment induces an oxidative stress state that enhances the inflammatory process mediated by adipokines secretion in HC.
topic	Chondrocytes Free fatty acids Inflammation Oxidative stress
url	http://link.springer.com/article/10.1186/s12944-017-0510-x
_version_	1612683949893484544

Hyperlipidemic microenvironment conditionates damage mechanisms in human chondrocytes by oxidative stress

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