Introductory microbiology lab skills and techniques in food science

Bibliographic Details
Main Authors: Shen, Gangqiang (Author), Zhang, Yifan (Author)
Format: Book
Language:English
Published: Oxford,United Kingdom : Elsevier Science ©2022
Subjects:
Table of Contents:
  • 1. Introduction of microbial and chemical safety and lab notebook record
  • 2. Practice of bright-field microscope
  • 3. Preparation of smears and simple stain practice
  • 4. Gram-stain practice
  • 5. Acid-fast stain practice
  • 6. Endospore stain practice
  • 7. Aseptic technique-bacteria transfer and streak plating
  • 8. Lab practice test 1-identify unknown bacteria using Gram-stain
  • 9. Introduction of bacteria medium, nutritional requirements (synthetic and complex media), selective & differential media (MacConkey, mannitol salt, blood agar)
  • 10. Lab practice test 2- dilution technology and quiz
  • 11. Numeration bacteria population by pour plating
  • 12. Numeration bacteria population by spread plating
  • 13. External conditions for bacterial growth (temperature, pH, oxygen, osmotic)
  • 14. Cultivation of anaerobic bacteria
  • 15. Biochemistry test of bacteria-1 (urease test, carbohydrate fermentation, catalase test, oxidase test)