Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD)
Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G 1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9U/ml) was determined: A 2(5) Central Composite Design (...
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| Format: | Article |
| Language: | English |
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Elsevier Limited
2005
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| Online Access: | http://eprints.utm.my/5574/ http://eprints.utm.my/5574/1/HananMoawiaIbrahim2005_OptimizationOfMedium.pdf |
| _version_ | 1848891086675640320 |
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| author | Ibrahim, Hanan Moawia Wan Yusoff, Wan Mokhtar Abdul Hamid, Aidil Md. Illias, Rosli Hassan, Othman Omar, Othman |
| author_facet | Ibrahim, Hanan Moawia Wan Yusoff, Wan Mokhtar Abdul Hamid, Aidil Md. Illias, Rosli Hassan, Othman Omar, Othman |
| author_sort | Ibrahim, Hanan Moawia |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G 1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9U/ml) was determined: A 2(5) Central Composite Design (CCD; half fraction) has been chosen to elucidate the combined effect of five process variables; tapioca starch, peptone, yeast extract, magnesium sulphate and potassium phosphate concentration. Concentrations of 4% tapioca starch, 2% peptone, 0.04% magnesium sulphate and 1 % sodium carbonate have been shown to be optimum for the production of CGTase. |
| first_indexed | 2025-11-15T20:52:23Z |
| format | Article |
| id | utm-5574 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:52:23Z |
| publishDate | 2005 |
| publisher | Elsevier Limited |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-55742017-08-22T01:35:54Z http://eprints.utm.my/5574/ Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD) Ibrahim, Hanan Moawia Wan Yusoff, Wan Mokhtar Abdul Hamid, Aidil Md. Illias, Rosli Hassan, Othman Omar, Othman T Technology (General) Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G 1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9U/ml) was determined: A 2(5) Central Composite Design (CCD; half fraction) has been chosen to elucidate the combined effect of five process variables; tapioca starch, peptone, yeast extract, magnesium sulphate and potassium phosphate concentration. Concentrations of 4% tapioca starch, 2% peptone, 0.04% magnesium sulphate and 1 % sodium carbonate have been shown to be optimum for the production of CGTase. Elsevier Limited 2005 Article PeerReviewed application/pdf en http://eprints.utm.my/5574/1/HananMoawiaIbrahim2005_OptimizationOfMedium.pdf Ibrahim, Hanan Moawia and Wan Yusoff, Wan Mokhtar and Abdul Hamid, Aidil and Md. Illias, Rosli and Hassan, Othman and Omar, Othman (2005) Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD). Process Biochemistry, 40 . pp. 753-758. ISSN 0032-9592 |
| spellingShingle | T Technology (General) Ibrahim, Hanan Moawia Wan Yusoff, Wan Mokhtar Abdul Hamid, Aidil Md. Illias, Rosli Hassan, Othman Omar, Othman Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD) |
| title | Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD) |
| title_full | Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD) |
| title_fullStr | Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD) |
| title_full_unstemmed | Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD) |
| title_short | Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD) |
| title_sort | optimization of medium for the production of β-cyclodextrin glucanotransferase using central composite design (ccd) |
| topic | T Technology (General) |
| url | http://eprints.utm.my/5574/ http://eprints.utm.my/5574/1/HananMoawiaIbrahim2005_OptimizationOfMedium.pdf |