Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae
Aminoglycosides include semi-synthetic and natural antibiotics isolated from Actinomycetes. They are well-known for their efficacy against the Enterobacteriaceae family. Nevertheless, the mass prescription of aminoglycosides in clinical settings has resulted in a significant rise in the number of am...
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| Format: | Final Year Project / Dissertation / Thesis |
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2022
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| Online Access: | http://eprints.utar.edu.my/4913/ http://eprints.utar.edu.my/4913/1/fyp_BM_2022_TCH.pdf |
| _version_ | 1848886276408737792 |
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| author | Theo, Chun Hao |
| author_facet | Theo, Chun Hao |
| author_sort | Theo, Chun Hao |
| building | UTAR Institutional Repository |
| collection | Online Access |
| description | Aminoglycosides include semi-synthetic and natural antibiotics isolated from Actinomycetes. They are well-known for their efficacy against the Enterobacteriaceae family. Nevertheless, the mass prescription of aminoglycosides in clinical settings has resulted in a significant rise in the number of aminoglycoside-resistant Enterobacteriaceae due to the presence of different resistance genes that synthesise aminoglycoside-modifying enzymes. In this study, a total of 60 clinical isolates of Enterobacteriaceae obtained from different hospitals in West Malaysia were subjected to eight antibiotics. Subsequently, the bacterial isolates were screened for the presence of ant(2’’)-Ia and aph(3’)-Ic genes simultaneously using duplex PCR. Twenty-three (38.33%) isolates of the 60 bacterial isolates were tested positive for ant(2’’)-Ia, 13 (21.67%) were tested positive for aph(3’)-Ic and only one (1.67%) bacterial isolate was tested positive for both ant(2’’)-Ic and aph(3’)-Ic genes. The prevalence of ant(2’’)-Ia and aph(3’)-Ic in Enterobacteriaceae was 40.00% and 23.33%, respectively. The ant(2’’)-Ia gene was more prevalent in Enterobacteriaceae as compared to the aph(3’)-Ic gene. Besides, E. coli and K. pneumoniae were found to host at least one aminoglycoside resistance gene. Statistical analysis determined that there were significant associations between the ant(2’’)-Ia gene with gentamicin, kanamycin, and imipenem resistance, and the aph(3’)-Ic gene with kanamycin resistance. However, the association between ant(2’’)-Ia gene with imipenem resistance may be caused by coincidence, in which the bacterial isolates hosting the ant(2’’)-Ia gene were carrying other imipenem resistance genes concurrently. This is because aminoglycoside resistance genes were proven to confer cross-resistance only within aminoglycosides, instead of other classes of antibiotics. Nonetheless, the age and gender of the patients have no statistically significant association with the resistance genes studied. |
| first_indexed | 2025-11-15T19:35:55Z |
| format | Final Year Project / Dissertation / Thesis |
| id | utar-4913 |
| institution | Universiti Tunku Abdul Rahman |
| institution_category | Local University |
| last_indexed | 2025-11-15T19:35:55Z |
| publishDate | 2022 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utar-49132022-12-30T09:25:14Z Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae Theo, Chun Hao Q Science (General) QD Chemistry Aminoglycosides include semi-synthetic and natural antibiotics isolated from Actinomycetes. They are well-known for their efficacy against the Enterobacteriaceae family. Nevertheless, the mass prescription of aminoglycosides in clinical settings has resulted in a significant rise in the number of aminoglycoside-resistant Enterobacteriaceae due to the presence of different resistance genes that synthesise aminoglycoside-modifying enzymes. In this study, a total of 60 clinical isolates of Enterobacteriaceae obtained from different hospitals in West Malaysia were subjected to eight antibiotics. Subsequently, the bacterial isolates were screened for the presence of ant(2’’)-Ia and aph(3’)-Ic genes simultaneously using duplex PCR. Twenty-three (38.33%) isolates of the 60 bacterial isolates were tested positive for ant(2’’)-Ia, 13 (21.67%) were tested positive for aph(3’)-Ic and only one (1.67%) bacterial isolate was tested positive for both ant(2’’)-Ic and aph(3’)-Ic genes. The prevalence of ant(2’’)-Ia and aph(3’)-Ic in Enterobacteriaceae was 40.00% and 23.33%, respectively. The ant(2’’)-Ia gene was more prevalent in Enterobacteriaceae as compared to the aph(3’)-Ic gene. Besides, E. coli and K. pneumoniae were found to host at least one aminoglycoside resistance gene. Statistical analysis determined that there were significant associations between the ant(2’’)-Ia gene with gentamicin, kanamycin, and imipenem resistance, and the aph(3’)-Ic gene with kanamycin resistance. However, the association between ant(2’’)-Ia gene with imipenem resistance may be caused by coincidence, in which the bacterial isolates hosting the ant(2’’)-Ia gene were carrying other imipenem resistance genes concurrently. This is because aminoglycoside resistance genes were proven to confer cross-resistance only within aminoglycosides, instead of other classes of antibiotics. Nonetheless, the age and gender of the patients have no statistically significant association with the resistance genes studied. 2022-05 Final Year Project / Dissertation / Thesis NonPeerReviewed application/pdf http://eprints.utar.edu.my/4913/1/fyp_BM_2022_TCH.pdf Theo, Chun Hao (2022) Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae. Final Year Project, UTAR. http://eprints.utar.edu.my/4913/ |
| spellingShingle | Q Science (General) QD Chemistry Theo, Chun Hao Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae |
| title | Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae |
| title_full | Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae |
| title_fullStr | Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae |
| title_full_unstemmed | Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae |
| title_short | Duplex PCR assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae |
| title_sort | duplex pcr assay for simultaneous detection of two aminoglycoside resistance genes in clinical samples of enterobacteriaceae |
| topic | Q Science (General) QD Chemistry |
| url | http://eprints.utar.edu.my/4913/ http://eprints.utar.edu.my/4913/1/fyp_BM_2022_TCH.pdf |