Salt-Induced Phase Separation Of Chaps And Potential Application For Hydrophobic Molecule Extraction
CHAPS is a zwitterionic detergent commonly utilized in protein biochemistry based applications. Despite widespread utility, understanding of CHAPS phase behaviour remained poor. In this study, the effect of salts on CHAPS phase behaviour was first investigated. Consolute solution temperature of C...
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| Format: | Thesis |
| Language: | English |
| Published: |
2019
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| Online Access: | http://eprints.usm.my/62295/ http://eprints.usm.my/62295/1/salt%20induced%20phase%20cut.pdf |
| _version_ | 1848884943573221376 |
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| author | Lian, Ai Ai |
| author_facet | Lian, Ai Ai |
| author_sort | Lian, Ai Ai |
| building | USM Institutional Repository |
| collection | Online Access |
| description | CHAPS is a zwitterionic detergent commonly utilized in protein biochemistry
based applications. Despite widespread utility, understanding of CHAPS phase
behaviour remained poor. In this study, the effect of salts on CHAPS phase behaviour
was first investigated. Consolute solution temperature of CHAPS-(NH4)2SO4 systems
were determined, followed by phase diagram construction. Potential of CHAPS-(NH4-
)2SO4 systems for hydrophobic molecule extraction via phase separation was then
evaluated using Coomassie Brilliant Blue (CBB) and recombinant cytochrome P450
(CYP) 2C19. Sulphate and phosphate salts were successful in inducing phase
separation of CHAPS. CHAPS and (NH4)2SO4 systems exhibited an upper consolute
solution temperature (UCST). CBB was found to partition extensively to micelle-rich
phase (MRP) of the 4.5% CHAPS and 8% (NH4)2SO4 system with an extraction yield
of 83±0.32%. Extraction yield of recombinant CYP 2C19 in MRP of the 13.5%
CHAPS and 10.8% (NH4)2SO4 system was 46±7.58%. The outcomes of this study
propound the scope for application of CHAPS as a component of phase separation
systems. |
| first_indexed | 2025-11-15T19:14:44Z |
| format | Thesis |
| id | usm-62295 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:14:44Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-622952025-05-26T02:43:36Z http://eprints.usm.my/62295/ Salt-Induced Phase Separation Of Chaps And Potential Application For Hydrophobic Molecule Extraction Lian, Ai Ai QD450-801 Physical and theoretical chemistry CHAPS is a zwitterionic detergent commonly utilized in protein biochemistry based applications. Despite widespread utility, understanding of CHAPS phase behaviour remained poor. In this study, the effect of salts on CHAPS phase behaviour was first investigated. Consolute solution temperature of CHAPS-(NH4)2SO4 systems were determined, followed by phase diagram construction. Potential of CHAPS-(NH4- )2SO4 systems for hydrophobic molecule extraction via phase separation was then evaluated using Coomassie Brilliant Blue (CBB) and recombinant cytochrome P450 (CYP) 2C19. Sulphate and phosphate salts were successful in inducing phase separation of CHAPS. CHAPS and (NH4)2SO4 systems exhibited an upper consolute solution temperature (UCST). CBB was found to partition extensively to micelle-rich phase (MRP) of the 4.5% CHAPS and 8% (NH4)2SO4 system with an extraction yield of 83±0.32%. Extraction yield of recombinant CYP 2C19 in MRP of the 13.5% CHAPS and 10.8% (NH4)2SO4 system was 46±7.58%. The outcomes of this study propound the scope for application of CHAPS as a component of phase separation systems. 2019-08 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/62295/1/salt%20induced%20phase%20cut.pdf Lian, Ai Ai (2019) Salt-Induced Phase Separation Of Chaps And Potential Application For Hydrophobic Molecule Extraction. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QD450-801 Physical and theoretical chemistry Lian, Ai Ai Salt-Induced Phase Separation Of Chaps And Potential Application For Hydrophobic Molecule Extraction |
| title | Salt-Induced Phase Separation Of
Chaps And Potential Application For
Hydrophobic Molecule Extraction |
| title_full | Salt-Induced Phase Separation Of
Chaps And Potential Application For
Hydrophobic Molecule Extraction |
| title_fullStr | Salt-Induced Phase Separation Of
Chaps And Potential Application For
Hydrophobic Molecule Extraction |
| title_full_unstemmed | Salt-Induced Phase Separation Of
Chaps And Potential Application For
Hydrophobic Molecule Extraction |
| title_short | Salt-Induced Phase Separation Of
Chaps And Potential Application For
Hydrophobic Molecule Extraction |
| title_sort | salt-induced phase separation of
chaps and potential application for
hydrophobic molecule extraction |
| topic | QD450-801 Physical and theoretical chemistry |
| url | http://eprints.usm.my/62295/ http://eprints.usm.my/62295/1/salt%20induced%20phase%20cut.pdf |