Annual meeting of the Japanese pharmacological society
High-performance liquid chromatography (HPLC) was compared with gas chromatography-mass spectrometry (GCMS) for determination of morphine in human urine. Calibration standards were prepared In human urine. Codeine and d3-morphine were added as the Internal standards for HPLC and GCMS. respectiv...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
2012
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| Online Access: | http://eprints.usm.my/54762/ http://eprints.usm.my/54762/1/DR%20RUZILAWATI%20ABU%20BAKAR-OCR.pdf |
| _version_ | 1848882895288008704 |
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| author | Bakar, Ruzilawati Abu |
| author_facet | Bakar, Ruzilawati Abu |
| author_sort | Bakar, Ruzilawati Abu |
| building | USM Institutional Repository |
| collection | Online Access |
| description | High-performance liquid chromatography (HPLC) was
compared with gas chromatography-mass spectrometry
(GCMS) for determination of morphine in human urine.
Calibration standards were prepared In human urine.
Codeine and d3-morphine were added as the Internal
standards for HPLC and GCMS. respectively. After solidphase
extraction, the reconstituted samples were divided
rnto aliquots for analysis br HPLC and GCMS. The
analytical performances o the two methods were
compared with regard to sensitivity, precision and
accuracy. Results Indicated that tfPlC analysis
produced results at least as precise, accurate and
specific as GCMS for morphrne. Thus, analytical results
by HPLC were comparable to GCMS. Therefore. HPLC
can be useful alternative to GCMS for measuring
morphine in urine. |
| first_indexed | 2025-11-15T18:42:11Z |
| format | Monograph |
| id | usm-54762 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:42:11Z |
| publishDate | 2012 |
| publisher | Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-547622022-09-19T07:06:34Z http://eprints.usm.my/54762/ Annual meeting of the Japanese pharmacological society Bakar, Ruzilawati Abu R Medicine High-performance liquid chromatography (HPLC) was compared with gas chromatography-mass spectrometry (GCMS) for determination of morphine in human urine. Calibration standards were prepared In human urine. Codeine and d3-morphine were added as the Internal standards for HPLC and GCMS. respectively. After solidphase extraction, the reconstituted samples were divided rnto aliquots for analysis br HPLC and GCMS. The analytical performances o the two methods were compared with regard to sensitivity, precision and accuracy. Results Indicated that tfPlC analysis produced results at least as precise, accurate and specific as GCMS for morphrne. Thus, analytical results by HPLC were comparable to GCMS. Therefore. HPLC can be useful alternative to GCMS for measuring morphine in urine. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2012 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/54762/1/DR%20RUZILAWATI%20ABU%20BAKAR-OCR.pdf Bakar, Ruzilawati Abu (2012) Annual meeting of the Japanese pharmacological society. Other. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine Bakar, Ruzilawati Abu Annual meeting of the Japanese pharmacological society |
| title | Annual meeting of the Japanese
pharmacological society |
| title_full | Annual meeting of the Japanese
pharmacological society |
| title_fullStr | Annual meeting of the Japanese
pharmacological society |
| title_full_unstemmed | Annual meeting of the Japanese
pharmacological society |
| title_short | Annual meeting of the Japanese
pharmacological society |
| title_sort | annual meeting of the japanese
pharmacological society |
| topic | R Medicine |
| url | http://eprints.usm.my/54762/ http://eprints.usm.my/54762/1/DR%20RUZILAWATI%20ABU%20BAKAR-OCR.pdf |