Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem’s Delight Orchid with Higher Efficiency
The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the production of transgenic Vanda Kasem’s Delight Tom Boykin (VKD) orchid. Several parameters such as PLB size, immersion period, level of wounding, Agrobacterium density, cocultiv...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://eprints.usm.my/38273/ http://eprints.usm.my/38273/1/Agrobacterium-Mediated_Transformation_of_the_Recalcitrant_Vanda_Kasem%27s_Delight_Orchid_with.pdf |
| _version_ | 1848878422402531328 |
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| author | Gnasekaran, Pavallekoodi Antony, Jessica Jeyanthi James Mohammad, Jasim Uddain Subramaniam, Sreeramanan |
| author_facet | Gnasekaran, Pavallekoodi Antony, Jessica Jeyanthi James Mohammad, Jasim Uddain Subramaniam, Sreeramanan |
| author_sort | Gnasekaran, Pavallekoodi |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the
production of transgenic Vanda Kasem’s Delight Tom Boykin (VKD) orchid. Several parameters such as PLB size, immersion
period, level of wounding, Agrobacterium density, cocultivation period, and concentration of acetosyringone were tested and
quantified using gusAgene expression to optimize the efficiency of Agrobacterium-mediated genetic transformation of VKD’s PLBs.
Based on the results, 3-4mm PLBs wounded by scalpel and immersed for 30 minutes in Agrobacterium suspension of 0.8 unit at
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| first_indexed | 2025-11-15T17:31:05Z |
| format | Article |
| id | usm-38273 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:31:05Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-382732020-05-06T06:46:14Z http://eprints.usm.my/38273/ Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem’s Delight Orchid with Higher Efficiency Gnasekaran, Pavallekoodi Antony, Jessica Jeyanthi James Mohammad, Jasim Uddain Subramaniam, Sreeramanan QH1 Natural history (General - Including nature conservation, geographical distribution) The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the production of transgenic Vanda Kasem’s Delight Tom Boykin (VKD) orchid. Several parameters such as PLB size, immersion period, level of wounding, Agrobacterium density, cocultivation period, and concentration of acetosyringone were tested and quantified using gusAgene expression to optimize the efficiency of Agrobacterium-mediated genetic transformation of VKD’s PLBs. Based on the results, 3-4mm PLBs wounded by scalpel and immersed for 30 minutes in Agrobacterium suspension of 0.8 unit at Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://eprints.usm.my/38273/1/Agrobacterium-Mediated_Transformation_of_the_Recalcitrant_Vanda_Kasem%27s_Delight_Orchid_with.pdf Gnasekaran, Pavallekoodi and Antony, Jessica Jeyanthi James and Mohammad, Jasim Uddain and Subramaniam, Sreeramanan (2014) Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem’s Delight Orchid with Higher Efficiency. Scientific World Journal, 2014 (583934). pp. 1-10. ISSN 2356-6140 http://dx.doi.org/10.1155/2014/583934 |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Gnasekaran, Pavallekoodi Antony, Jessica Jeyanthi James Mohammad, Jasim Uddain Subramaniam, Sreeramanan Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem’s Delight Orchid with Higher Efficiency |
| title | Agrobacterium-Mediated Transformation of the Recalcitrant
Vanda Kasem’s Delight Orchid with Higher Efficiency |
| title_full | Agrobacterium-Mediated Transformation of the Recalcitrant
Vanda Kasem’s Delight Orchid with Higher Efficiency |
| title_fullStr | Agrobacterium-Mediated Transformation of the Recalcitrant
Vanda Kasem’s Delight Orchid with Higher Efficiency |
| title_full_unstemmed | Agrobacterium-Mediated Transformation of the Recalcitrant
Vanda Kasem’s Delight Orchid with Higher Efficiency |
| title_short | Agrobacterium-Mediated Transformation of the Recalcitrant
Vanda Kasem’s Delight Orchid with Higher Efficiency |
| title_sort | agrobacterium-mediated transformation of the recalcitrant
vanda kasem’s delight orchid with higher efficiency |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/38273/ http://eprints.usm.my/38273/ http://eprints.usm.my/38273/1/Agrobacterium-Mediated_Transformation_of_the_Recalcitrant_Vanda_Kasem%27s_Delight_Orchid_with.pdf |