Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats
The effects of nicotine administration on structural and cellular parameters of bone histomorphometry, cotinine, and biomarkers of bone remodeling were studied in twenty-one Sprague-Dawley male rats. Rats aged three months and weighing between 250-300 g were divided into three groups. Group 1 was...
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| Format: | Article |
| Language: | English |
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Microscopy Society of Singapore
2007
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| Online Access: | http://eprints.usm.my/35857/ http://eprints.usm.my/35857/1/Page_10-24.pdf |
| _version_ | 1848877739951521792 |
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| author | H, Hermizi O, Faizah S Ima, Nirwana S Ahmad, Nazrun DA , Luke M, Norazlina |
| author_facet | H, Hermizi O, Faizah S Ima, Nirwana S Ahmad, Nazrun DA , Luke M, Norazlina |
| author_sort | H, Hermizi |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The effects of nicotine administration on structural and cellular parameters of bone
histomorphometry, cotinine, and biomarkers of bone remodeling were studied in twenty-one
Sprague-Dawley male rats. Rats aged three months and weighing between 250-300 g were divided
into three groups. Group 1 was the baseline group, which was sacrificed without treatment. The
other 2 groups were the control group and the nicotine group. The nicotine group was treated with
nicotine 7 mg/kg body weight and the C group was treated with normal saline only. Treatment was
given by intraperitoneal injection, six days a week for a period of 4 months. Histomorphometric
analysis was done on the metaphyseal region of the trabecular bone of the left femur by using
an image analyzer. Biochemical analysis was done using ELISA-test kit to compare the serum
cotinine, osteocalcin and pyridinoline (PYD) levels between pretreatment and after 4 months
treatment in the control and nicotine groups. Histomorphometric analysis revealed that nicotine
significantly decreased the trabecular bone volume (BV/TV) and the osteoblast surface (Ob.S/BS),
and increased the osteoclast surface (Oc.S/BS) and the eroded surface (ES/BS) compared to the
baseline and control groups. In addition, biochemical analysis showed that nicotine treatment
for 4 months significantly decreased the osteocalcin (bone formation marker) levels while the
cotinine and PYD (bone resorption marker) levels were increased as compared to pretreatment.
We concluded that treatment with nicotine 7 mg/kg for 4 months exerted negative a effect on the
trabecular bone histomorphometric parameters and bone remodeling biomarkers. |
| first_indexed | 2025-11-15T17:20:14Z |
| format | Article |
| id | usm-35857 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T17:20:14Z |
| publishDate | 2007 |
| publisher | Microscopy Society of Singapore |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-358572017-07-26T00:30:10Z http://eprints.usm.my/35857/ Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats H, Hermizi O, Faizah S Ima, Nirwana S Ahmad, Nazrun DA , Luke M, Norazlina RA0421 Public health. Hygiene. Preventive Medicine The effects of nicotine administration on structural and cellular parameters of bone histomorphometry, cotinine, and biomarkers of bone remodeling were studied in twenty-one Sprague-Dawley male rats. Rats aged three months and weighing between 250-300 g were divided into three groups. Group 1 was the baseline group, which was sacrificed without treatment. The other 2 groups were the control group and the nicotine group. The nicotine group was treated with nicotine 7 mg/kg body weight and the C group was treated with normal saline only. Treatment was given by intraperitoneal injection, six days a week for a period of 4 months. Histomorphometric analysis was done on the metaphyseal region of the trabecular bone of the left femur by using an image analyzer. Biochemical analysis was done using ELISA-test kit to compare the serum cotinine, osteocalcin and pyridinoline (PYD) levels between pretreatment and after 4 months treatment in the control and nicotine groups. Histomorphometric analysis revealed that nicotine significantly decreased the trabecular bone volume (BV/TV) and the osteoblast surface (Ob.S/BS), and increased the osteoclast surface (Oc.S/BS) and the eroded surface (ES/BS) compared to the baseline and control groups. In addition, biochemical analysis showed that nicotine treatment for 4 months significantly decreased the osteocalcin (bone formation marker) levels while the cotinine and PYD (bone resorption marker) levels were increased as compared to pretreatment. We concluded that treatment with nicotine 7 mg/kg for 4 months exerted negative a effect on the trabecular bone histomorphometric parameters and bone remodeling biomarkers. Microscopy Society of Singapore 2007-04 Article PeerReviewed application/pdf en http://eprints.usm.my/35857/1/Page_10-24.pdf H, Hermizi and O, Faizah and S Ima, Nirwana and S Ahmad, Nazrun and DA , Luke and M, Norazlina (2007) Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats. Annals of Microscopy , 7. pp. 10-24. ISSN 0219-2209 |
| spellingShingle | RA0421 Public health. Hygiene. Preventive Medicine H, Hermizi O, Faizah S Ima, Nirwana S Ahmad, Nazrun DA , Luke M, Norazlina Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats |
| title | Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats |
| title_full | Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats |
| title_fullStr | Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats |
| title_full_unstemmed | Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats |
| title_short | Nicotine Impaired Bone Histomorphometric Parameters And Bone Remodeling Biomarkers In Sprague-Dawley Male Rats |
| title_sort | nicotine impaired bone histomorphometric parameters and bone remodeling biomarkers in sprague-dawley male rats |
| topic | RA0421 Public health. Hygiene. Preventive Medicine |
| url | http://eprints.usm.my/35857/ http://eprints.usm.my/35857/1/Page_10-24.pdf |