Elucidation of Musa acuminata cv. Berangan root infection by FOC (Tropical Race 4) by RNA sequencing and analysis
Musa acuminata cv. Berangan (AAA) is a type of banana locally grown in Malaysia. These bananas as well as Musa acuminata cv. Cavendish (AAA) are also facing a major threat from a typical soil borne fungus identified as Fusarium oxysporum f. sp. cubense race 4 (FocR4). Its characteristics as a comple...
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| Format: | Article |
| Language: | English |
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Pelagia Research Library
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/97065/ http://psasir.upm.edu.my/id/eprint/97065/1/ABSTRACT.pdf |
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| author | Munusamy, Umaiyal Zaidi, Kamilatulhusna Baharum, Nadiya Akmal Ho, Hui Li Mohd Yusuf, Yusmin Othman, Rofina Yasmin |
| author_facet | Munusamy, Umaiyal Zaidi, Kamilatulhusna Baharum, Nadiya Akmal Ho, Hui Li Mohd Yusuf, Yusmin Othman, Rofina Yasmin |
| author_sort | Munusamy, Umaiyal |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Musa acuminata cv. Berangan (AAA) is a type of banana locally grown in Malaysia. These bananas as well as Musa acuminata cv. Cavendish (AAA) are also facing a major threat from a typical soil borne fungus identified as Fusarium oxysporum f. sp. cubense race 4 (FocR4). Its characteristics as a complex pathogen manifesting as subtypes or races are the main reasons its infections are difficult to control. Genome sequence availability of the double haploid Musa acuminata originating from Pahang has become very useful to analyse RNA-seq reads and to identify the transcriptome profile of the host response between different groups was accomplished using RNA-Seq technology based on the Illumina HiSeqTm 2000 platform. Three sets of libraries derived from infected and mock infected plants (experimental groups) between different time points (0, 48, 96 h) shows over forty million reads were generated, each corresponding to coverage of >4,000,000,000 to <8,000,000,000 bases. About 0.10-66% reads were mapped to Musa acuminata DH Pahang genome sequence. This study provides the statistical analysis of the sequence reads. Based on this information, further analysis on gene expression patterns influenced by Foc race infection within the tested groups and time points will help in the understanding of the host pathogenic responses. In future discovery on many new genes for diagnosis of plant infection could be achieved through excessive transcriptomic data. |
| first_indexed | 2025-11-15T13:18:08Z |
| format | Article |
| id | upm-97065 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:18:08Z |
| publishDate | 2021 |
| publisher | Pelagia Research Library |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-970652022-10-17T01:49:48Z http://psasir.upm.edu.my/id/eprint/97065/ Elucidation of Musa acuminata cv. Berangan root infection by FOC (Tropical Race 4) by RNA sequencing and analysis Munusamy, Umaiyal Zaidi, Kamilatulhusna Baharum, Nadiya Akmal Ho, Hui Li Mohd Yusuf, Yusmin Othman, Rofina Yasmin Musa acuminata cv. Berangan (AAA) is a type of banana locally grown in Malaysia. These bananas as well as Musa acuminata cv. Cavendish (AAA) are also facing a major threat from a typical soil borne fungus identified as Fusarium oxysporum f. sp. cubense race 4 (FocR4). Its characteristics as a complex pathogen manifesting as subtypes or races are the main reasons its infections are difficult to control. Genome sequence availability of the double haploid Musa acuminata originating from Pahang has become very useful to analyse RNA-seq reads and to identify the transcriptome profile of the host response between different groups was accomplished using RNA-Seq technology based on the Illumina HiSeqTm 2000 platform. Three sets of libraries derived from infected and mock infected plants (experimental groups) between different time points (0, 48, 96 h) shows over forty million reads were generated, each corresponding to coverage of >4,000,000,000 to <8,000,000,000 bases. About 0.10-66% reads were mapped to Musa acuminata DH Pahang genome sequence. This study provides the statistical analysis of the sequence reads. Based on this information, further analysis on gene expression patterns influenced by Foc race infection within the tested groups and time points will help in the understanding of the host pathogenic responses. In future discovery on many new genes for diagnosis of plant infection could be achieved through excessive transcriptomic data. Pelagia Research Library 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97065/1/ABSTRACT.pdf Munusamy, Umaiyal and Zaidi, Kamilatulhusna and Baharum, Nadiya Akmal and Ho, Hui Li and Mohd Yusuf, Yusmin and Othman, Rofina Yasmin (2021) Elucidation of Musa acuminata cv. Berangan root infection by FOC (Tropical Race 4) by RNA sequencing and analysis. Asian Journal of Plant Science and Research, 11 (3). 96 - 112. ISSN 2249-7412 https://www.imedpub.com/abstract/elucidation-of-musa-acuminata-cv-berangan-root-infection-by-foc-tropical-race-4-by-rna-sequencing-and-analysis-35686.html |
| spellingShingle | Munusamy, Umaiyal Zaidi, Kamilatulhusna Baharum, Nadiya Akmal Ho, Hui Li Mohd Yusuf, Yusmin Othman, Rofina Yasmin Elucidation of Musa acuminata cv. Berangan root infection by FOC (Tropical Race 4) by RNA sequencing and analysis |
| title | Elucidation of Musa acuminata cv. Berangan root infection by FOC (Tropical Race 4) by RNA sequencing and analysis |
| title_full | Elucidation of Musa acuminata cv. Berangan root infection by FOC (Tropical Race 4) by RNA sequencing and analysis |
| title_fullStr | Elucidation of Musa acuminata cv. Berangan root infection by FOC (Tropical Race 4) by RNA sequencing and analysis |
| title_full_unstemmed | Elucidation of Musa acuminata cv. Berangan root infection by FOC (Tropical Race 4) by RNA sequencing and analysis |
| title_short | Elucidation of Musa acuminata cv. Berangan root infection by FOC (Tropical Race 4) by RNA sequencing and analysis |
| title_sort | elucidation of musa acuminata cv. berangan root infection by foc (tropical race 4) by rna sequencing and analysis |
| url | http://psasir.upm.edu.my/id/eprint/97065/ http://psasir.upm.edu.my/id/eprint/97065/ http://psasir.upm.edu.my/id/eprint/97065/1/ABSTRACT.pdf |