The effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation
Corynebacterium pseudotuberculosis is a Gram-positive pathogen that commonly causes caseous lymphadenitis which occurs in sheep, goats, cattle, buffalo and horses. This disease has long been shown to be a major cause of economic loss on sheep industries. Dimethyl sulfoxide (DMSO) is known to be effe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Institute of Physics Publishing
2021
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/95385/ |
| _version_ | 1848862145774616576 |
|---|---|
| author | Yaacob, Mohamad Fakhri Abdullah, Faez Firdaus Jesse Mohamad Jamil, Norashirene Mohamed Yunus, Norfatimah Aazmi, Shafiq Raja Yahya, Mohd Fakharul Zaman |
| author_facet | Yaacob, Mohamad Fakhri Abdullah, Faez Firdaus Jesse Mohamad Jamil, Norashirene Mohamed Yunus, Norfatimah Aazmi, Shafiq Raja Yahya, Mohd Fakharul Zaman |
| author_sort | Yaacob, Mohamad Fakhri |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Corynebacterium pseudotuberculosis is a Gram-positive pathogen that commonly causes caseous lymphadenitis which occurs in sheep, goats, cattle, buffalo and horses. This disease has long been shown to be a major cause of economic loss on sheep industries. Dimethyl sulfoxide (DMSO) is known to be effective against a wide spectrum of pathogens however, its efficacy against C. pseudotuberculosis biofilm remains uncertain. The objective of this study was to predict the antibiofilm potential of DMSO against C. pseudotuberculosis using in silico protein interaction network analysis and experimentally determine the antibiofilm activity using standard microplate assay system. As compared to the protein interaction network of S. typhimurium biofilm that had previously been shown to be inhibited by DMSO, the protein interaction network of C. pseudotuberculosis showed similar nodes, hub proteins and functional linkages between glycolytic enzymes. Further experimental validation revealed that the treatment with DMSO significantly (p<0.05) inhibited C. pseudotuberculosis biofilm at all tested concentrations (1.56% - 50%). The findings from the present study suggest the potential application of DMSO in controlling caseous lymphadenitis in ruminants. |
| first_indexed | 2025-11-15T13:12:22Z |
| format | Article |
| id | upm-95385 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:12:22Z |
| publishDate | 2021 |
| publisher | Institute of Physics Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-953852023-02-03T08:41:22Z http://psasir.upm.edu.my/id/eprint/95385/ The effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation Yaacob, Mohamad Fakhri Abdullah, Faez Firdaus Jesse Mohamad Jamil, Norashirene Mohamed Yunus, Norfatimah Aazmi, Shafiq Raja Yahya, Mohd Fakharul Zaman Corynebacterium pseudotuberculosis is a Gram-positive pathogen that commonly causes caseous lymphadenitis which occurs in sheep, goats, cattle, buffalo and horses. This disease has long been shown to be a major cause of economic loss on sheep industries. Dimethyl sulfoxide (DMSO) is known to be effective against a wide spectrum of pathogens however, its efficacy against C. pseudotuberculosis biofilm remains uncertain. The objective of this study was to predict the antibiofilm potential of DMSO against C. pseudotuberculosis using in silico protein interaction network analysis and experimentally determine the antibiofilm activity using standard microplate assay system. As compared to the protein interaction network of S. typhimurium biofilm that had previously been shown to be inhibited by DMSO, the protein interaction network of C. pseudotuberculosis showed similar nodes, hub proteins and functional linkages between glycolytic enzymes. Further experimental validation revealed that the treatment with DMSO significantly (p<0.05) inhibited C. pseudotuberculosis biofilm at all tested concentrations (1.56% - 50%). The findings from the present study suggest the potential application of DMSO in controlling caseous lymphadenitis in ruminants. Institute of Physics Publishing 2021 Article PeerReviewed Yaacob, Mohamad Fakhri and Abdullah, Faez Firdaus Jesse and Mohamad Jamil, Norashirene and Mohamed Yunus, Norfatimah and Aazmi, Shafiq and Raja Yahya, Mohd Fakharul Zaman (2021) The effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation. Journal of Physics: Conference Series, 1874 (1). pp. 1-10. ISSN 1742-6588; ESSN: 1742-6596 https://iopscience.iop.org/article/10.1088/1742-6596/1874/1/012055/meta 10.1088/1742-6596/1874/1/012055 |
| spellingShingle | Yaacob, Mohamad Fakhri Abdullah, Faez Firdaus Jesse Mohamad Jamil, Norashirene Mohamed Yunus, Norfatimah Aazmi, Shafiq Raja Yahya, Mohd Fakharul Zaman The effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation |
| title | The effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation |
| title_full | The effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation |
| title_fullStr | The effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation |
| title_full_unstemmed | The effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation |
| title_short | The effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation |
| title_sort | effect of dimethyl sulfoxide on corynebacterium pseudotuberculosis biofilm: an in silico prediction and experimental validation |
| url | http://psasir.upm.edu.my/id/eprint/95385/ http://psasir.upm.edu.my/id/eprint/95385/ http://psasir.upm.edu.my/id/eprint/95385/ |