Sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization

This study evaluates the process conditions, (enzyme concentration (120-1200 U/g substrate), temperature (30-60 °C), and pH (3-9)) of enzymatic hydrolysis (EH) for sugar recovery from leftover croissants (LC) and leftover doughnut (LD), and characterizing its residue and hydrolysate. The highest sug...

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Main Authors: Mohd Thani, Nurfatimah, Mustapa Kamal, Siti Mazlina, Taip, Farah Saleena, Sulaiman, Alifdalino, Omar, Rozita
Format: Article
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:http://psasir.upm.edu.my/id/eprint/95210/
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author Mohd Thani, Nurfatimah
Mustapa Kamal, Siti Mazlina
Taip, Farah Saleena
Sulaiman, Alifdalino
Omar, Rozita
author_facet Mohd Thani, Nurfatimah
Mustapa Kamal, Siti Mazlina
Taip, Farah Saleena
Sulaiman, Alifdalino
Omar, Rozita
author_sort Mohd Thani, Nurfatimah
building UPM Institutional Repository
collection Online Access
description This study evaluates the process conditions, (enzyme concentration (120-1200 U/g substrate), temperature (30-60 °C), and pH (3-9)) of enzymatic hydrolysis (EH) for sugar recovery from leftover croissants (LC) and leftover doughnut (LD), and characterizing its residue and hydrolysate. The highest sugar yield recovered from LC was 574.21 ± 0.74 mg/g (840 U/g substrate, 49 °C and pH 3) and for LD was 460.53 ± 0.74 mg/g (1176 U/g substrate, 47 °C and pH 3). The highest fructose and glucose yield for LC and LD were 14.47±0.73 mg/g and 11.84±0.21 mg/g, and 13.26±0.63 mg/g and 10.34±0.11 mg/g, respectively. Morphology analysis (SEM) showed that the structure of LC and LD had changes in its starch granules that indicates hydrolysis process occurrence. The presence of monosaccharides and oligosaccharides were detected from FTIR. HMF was also detected from sugar degradation due to EH, (0.043 ± 0.0334 mg/g for LC) and (0.023 ± 0.0124 mg/g for LD).
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institution Universiti Putra Malaysia
institution_category Local University
last_indexed 2025-11-15T13:11:38Z
publishDate 2021
publisher Penerbit Universiti Kebangsaan Malaysia
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spelling upm-952102023-04-06T04:02:10Z http://psasir.upm.edu.my/id/eprint/95210/ Sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization Mohd Thani, Nurfatimah Mustapa Kamal, Siti Mazlina Taip, Farah Saleena Sulaiman, Alifdalino Omar, Rozita This study evaluates the process conditions, (enzyme concentration (120-1200 U/g substrate), temperature (30-60 °C), and pH (3-9)) of enzymatic hydrolysis (EH) for sugar recovery from leftover croissants (LC) and leftover doughnut (LD), and characterizing its residue and hydrolysate. The highest sugar yield recovered from LC was 574.21 ± 0.74 mg/g (840 U/g substrate, 49 °C and pH 3) and for LD was 460.53 ± 0.74 mg/g (1176 U/g substrate, 47 °C and pH 3). The highest fructose and glucose yield for LC and LD were 14.47±0.73 mg/g and 11.84±0.21 mg/g, and 13.26±0.63 mg/g and 10.34±0.11 mg/g, respectively. Morphology analysis (SEM) showed that the structure of LC and LD had changes in its starch granules that indicates hydrolysis process occurrence. The presence of monosaccharides and oligosaccharides were detected from FTIR. HMF was also detected from sugar degradation due to EH, (0.043 ± 0.0334 mg/g for LC) and (0.023 ± 0.0124 mg/g for LD). Penerbit Universiti Kebangsaan Malaysia 2021 Article PeerReviewed Mohd Thani, Nurfatimah and Mustapa Kamal, Siti Mazlina and Taip, Farah Saleena and Sulaiman, Alifdalino and Omar, Rozita (2021) Sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization. Sains Malaysiana, 50 (10). 2977 - 2991. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil10_2021/Jilid50Bil10_2021ms2977-2991.html 10.17576/jsm-2021-5010-12
spellingShingle Mohd Thani, Nurfatimah
Mustapa Kamal, Siti Mazlina
Taip, Farah Saleena
Sulaiman, Alifdalino
Omar, Rozita
Sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization
title Sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization
title_full Sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization
title_fullStr Sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization
title_full_unstemmed Sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization
title_short Sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization
title_sort sugar rcovery from bakery leftovers through enzymatic hydrolysis: effect of process conditions and product characterization
url http://psasir.upm.edu.my/id/eprint/95210/
http://psasir.upm.edu.my/id/eprint/95210/
http://psasir.upm.edu.my/id/eprint/95210/