Improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against UV light
In order to improve the membrane lipophilicity and the affinity towards the environment of lipid bilayers, squalene (SQ) could be conjugated to phospholipids in the formation of liposomes. The effect of membrane composition and concentrations on the degradation of liposomes prepared via the extrusio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89327/ http://psasir.upm.edu.my/id/eprint/89327/1/UV.pdf |
| _version_ | 1848860826819100672 |
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| author | Toopkanloo, Sahar Pakbaten Tan, Tai Boon Abas, Faridah Azam, Mohammad Nehdi, Imededdine Arbi Tan, Chin Ping |
| author_facet | Toopkanloo, Sahar Pakbaten Tan, Tai Boon Abas, Faridah Azam, Mohammad Nehdi, Imededdine Arbi Tan, Chin Ping |
| author_sort | Toopkanloo, Sahar Pakbaten |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In order to improve the membrane lipophilicity and the affinity towards the environment of lipid bilayers, squalene (SQ) could be conjugated to phospholipids in the formation of liposomes. The effect of membrane composition and concentrations on the degradation of liposomes prepared via the extrusion method was investigated. Liposomes were prepared using a mixture of SQ, cholesterol (CH) and Tween80 (TW80). Based on the optimal conditions, liposome batches were prepared in the absence and presence of SQ. Their physicochemical and stability behavior were evaluated as a function of liposome constituent. From the optimization study, the liposomal formulation containing 5% (w/w) mixed soy lecithin (ML), 0.5% (w/w) SQ, 0.3% (w/w) CH and 0.75% (w/w) TW80 had optimal physicochemical properties and displayed a unilamellar structure. Liposome prepared using the optimal formulation had a low particle size (158.31 ± 2.96 nm) and acceptable %increase in the particle size (15.09% ± 3.76%) and %trolox equivalent antioxidant capacity (%TEAC) loss (35.69% ± 0.72%) against UV light treatment (280–320 nm) for 6 h. The interesting outcome of this research was the association of naturally occurring substance SQ for size reduction without the extra input of energy or mechanical procedures, and improvement of vesicle stability and antioxidant activity of ML-based liposome. This study also demonstrated that the presence of SQ in the membrane might increase the acyl chain dynamics and decrease the viscosity of the dispersion, thereby limiting long-term stability of the liposome. |
| first_indexed | 2025-11-15T12:51:25Z |
| format | Article |
| id | upm-89327 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:51:25Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-893272021-08-19T23:07:46Z http://psasir.upm.edu.my/id/eprint/89327/ Improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against UV light Toopkanloo, Sahar Pakbaten Tan, Tai Boon Abas, Faridah Azam, Mohammad Nehdi, Imededdine Arbi Tan, Chin Ping In order to improve the membrane lipophilicity and the affinity towards the environment of lipid bilayers, squalene (SQ) could be conjugated to phospholipids in the formation of liposomes. The effect of membrane composition and concentrations on the degradation of liposomes prepared via the extrusion method was investigated. Liposomes were prepared using a mixture of SQ, cholesterol (CH) and Tween80 (TW80). Based on the optimal conditions, liposome batches were prepared in the absence and presence of SQ. Their physicochemical and stability behavior were evaluated as a function of liposome constituent. From the optimization study, the liposomal formulation containing 5% (w/w) mixed soy lecithin (ML), 0.5% (w/w) SQ, 0.3% (w/w) CH and 0.75% (w/w) TW80 had optimal physicochemical properties and displayed a unilamellar structure. Liposome prepared using the optimal formulation had a low particle size (158.31 ± 2.96 nm) and acceptable %increase in the particle size (15.09% ± 3.76%) and %trolox equivalent antioxidant capacity (%TEAC) loss (35.69% ± 0.72%) against UV light treatment (280–320 nm) for 6 h. The interesting outcome of this research was the association of naturally occurring substance SQ for size reduction without the extra input of energy or mechanical procedures, and improvement of vesicle stability and antioxidant activity of ML-based liposome. This study also demonstrated that the presence of SQ in the membrane might increase the acyl chain dynamics and decrease the viscosity of the dispersion, thereby limiting long-term stability of the liposome. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89327/1/UV.pdf Toopkanloo, Sahar Pakbaten and Tan, Tai Boon and Abas, Faridah and Azam, Mohammad and Nehdi, Imededdine Arbi and Tan, Chin Ping (2020) Improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against UV light. Molecules, 25 (24). pp. 1-24. ISSN 1420-3049 https://www.mdpi.com/1420-3049/25/24/5873 10.3390/molecules25245873 |
| spellingShingle | Toopkanloo, Sahar Pakbaten Tan, Tai Boon Abas, Faridah Azam, Mohammad Nehdi, Imededdine Arbi Tan, Chin Ping Improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against UV light |
| title | Improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against UV light |
| title_full | Improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against UV light |
| title_fullStr | Improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against UV light |
| title_full_unstemmed | Improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against UV light |
| title_short | Improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against UV light |
| title_sort | improving vesicular integrity and antioxidant activity of novel mixed soy lecithin-based liposomes containing squalene and their stability against uv light |
| url | http://psasir.upm.edu.my/id/eprint/89327/ http://psasir.upm.edu.my/id/eprint/89327/ http://psasir.upm.edu.my/id/eprint/89327/ http://psasir.upm.edu.my/id/eprint/89327/1/UV.pdf |