Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review
5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and en...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor and Francis
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87877/ http://psasir.upm.edu.my/id/eprint/87877/1/ABSTRACT.pdf |
| _version_ | 1848860518481133568 |
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| author | Semail, Nadhiratul farihin Abdul Keyon, Aemi Syazwani Saad, Bahruddin Noordin, Siti Salmah Nik Mohamed Kamal, Nik Nur Syazni Mohamad Zain, Nur Nadhirah Azizi, Juzaili Kamaruzaman, Sazlinda Yahaya, Noorfatimah |
| author_facet | Semail, Nadhiratul farihin Abdul Keyon, Aemi Syazwani Saad, Bahruddin Noordin, Siti Salmah Nik Mohamed Kamal, Nik Nur Syazni Mohamad Zain, Nur Nadhirah Azizi, Juzaili Kamaruzaman, Sazlinda Yahaya, Noorfatimah |
| author_sort | Semail, Nadhiratul farihin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | 5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and encourages fundamental investigations of this medication. The development of accurate and reliable sample preparation, as well as analytical methods, is critical to isolate targeted analytes from complex matrices, apart from increasing detection sensitivity of analytes. With that, this paper presents a review of prior studies pertaining to chromatographic and electrophoretic methods that focused on the analysis of 5-FU and its metabolites in biological matrices such as plasma and urine. This paper concentrates on HPLC, GC and CE systems, which are the most commonly used strategies for analytical separation of 5-FU and its metabolites from samples. Detection of these antineoplastic agents at trace level demands highly sensitive and selective analytical methodologies. Application of these analytical techniques to biological matrices is reviewed with a focus on method development strategies, including types of mobile phases and background electrolytes employed in LC and CE systems. |
| first_indexed | 2025-11-15T12:46:31Z |
| format | Article |
| id | upm-87877 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:46:31Z |
| publishDate | 2020 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-878772022-06-01T00:56:52Z http://psasir.upm.edu.my/id/eprint/87877/ Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review Semail, Nadhiratul farihin Abdul Keyon, Aemi Syazwani Saad, Bahruddin Noordin, Siti Salmah Nik Mohamed Kamal, Nik Nur Syazni Mohamad Zain, Nur Nadhirah Azizi, Juzaili Kamaruzaman, Sazlinda Yahaya, Noorfatimah 5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and encourages fundamental investigations of this medication. The development of accurate and reliable sample preparation, as well as analytical methods, is critical to isolate targeted analytes from complex matrices, apart from increasing detection sensitivity of analytes. With that, this paper presents a review of prior studies pertaining to chromatographic and electrophoretic methods that focused on the analysis of 5-FU and its metabolites in biological matrices such as plasma and urine. This paper concentrates on HPLC, GC and CE systems, which are the most commonly used strategies for analytical separation of 5-FU and its metabolites from samples. Detection of these antineoplastic agents at trace level demands highly sensitive and selective analytical methodologies. Application of these analytical techniques to biological matrices is reviewed with a focus on method development strategies, including types of mobile phases and background electrolytes employed in LC and CE systems. Taylor and Francis 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87877/1/ABSTRACT.pdf Semail, Nadhiratul farihin and Abdul Keyon, Aemi Syazwani and Saad, Bahruddin and Noordin, Siti Salmah and Nik Mohamed Kamal, Nik Nur Syazni and Mohamad Zain, Nur Nadhirah and Azizi, Juzaili and Kamaruzaman, Sazlinda and Yahaya, Noorfatimah (2020) Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review. Journal of Liquid Chromatography and Related Technologies, 43 (15-16). 562 - 579. ISSN 1082-6076; ESSN: 1520-572X https://www.tandfonline.com/doi/abs/10.1080/10826076.2020.1781654 10.1080/10826076.2020.1781654 |
| spellingShingle | Semail, Nadhiratul farihin Abdul Keyon, Aemi Syazwani Saad, Bahruddin Noordin, Siti Salmah Nik Mohamed Kamal, Nik Nur Syazni Mohamad Zain, Nur Nadhirah Azizi, Juzaili Kamaruzaman, Sazlinda Yahaya, Noorfatimah Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review |
| title | Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review |
| title_full | Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review |
| title_fullStr | Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review |
| title_full_unstemmed | Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review |
| title_short | Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review |
| title_sort | analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review |
| url | http://psasir.upm.edu.my/id/eprint/87877/ http://psasir.upm.edu.my/id/eprint/87877/ http://psasir.upm.edu.my/id/eprint/87877/ http://psasir.upm.edu.my/id/eprint/87877/1/ABSTRACT.pdf |