Cloning of human- interleukin-12 into pJET cloning vector for anti-cancer vaccine development
The Newcastle disease virus can replicate rapidly and kill human cancer cells. Therefore it has the potential to be developed as a cancer vaccine. However, the immune system hinders the replication of the virus in these cells. In order to make it more efficient as a cancer vaccine, the the human int...
| Main Author: | |
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/85123/ http://psasir.upm.edu.my/id/eprint/85123/1/FBSB%202015%20112%20-%20IR.pdf |
| _version_ | 1848859999693963264 |
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| author | Che Ani, Muhamad Alhapis |
| author_facet | Che Ani, Muhamad Alhapis |
| author_sort | Che Ani, Muhamad Alhapis |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The Newcastle disease virus can replicate rapidly and kill human cancer cells. Therefore it has the potential to be developed as a cancer vaccine. However, the immune system hinders the replication of the virus in these cells. In order to make it more efficient as a cancer vaccine, the the human interleukin-12 (hIL-12) gene will be cloned into the virus. However, before this gene can be transferred to the virus, it must first be cloned into a cloning vector. In this project, the hIL-12 gene was obtained from the plasmid pUNO-hIL12 and cloned into a pJET cloning vector. A set of forward and reverse primers with NheI restriction included was designed to amplify hIL-12 gene. This will lead to producing the hIL-12 gene fragment with NheI restriction sites after amplification by polymerase chain reaction (PCR) cycles. The PCR product was then purified by using MEGAquick-spinTM Total Fragment DNA Purification Kit. The purified hIL-12 was then cloned into pJET cloning vector by T4 DNA ligase and transformed into competent Escherichia. coli Top 10 cells by using heat-shock transformation method. The cloning was verified using NheI restriction enzyme digestion analysis and further confirmed by sequencing. |
| first_indexed | 2025-11-15T12:38:16Z |
| format | Project Paper Report |
| id | upm-85123 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:38:16Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-851232021-12-27T07:55:23Z http://psasir.upm.edu.my/id/eprint/85123/ Cloning of human- interleukin-12 into pJET cloning vector for anti-cancer vaccine development Che Ani, Muhamad Alhapis The Newcastle disease virus can replicate rapidly and kill human cancer cells. Therefore it has the potential to be developed as a cancer vaccine. However, the immune system hinders the replication of the virus in these cells. In order to make it more efficient as a cancer vaccine, the the human interleukin-12 (hIL-12) gene will be cloned into the virus. However, before this gene can be transferred to the virus, it must first be cloned into a cloning vector. In this project, the hIL-12 gene was obtained from the plasmid pUNO-hIL12 and cloned into a pJET cloning vector. A set of forward and reverse primers with NheI restriction included was designed to amplify hIL-12 gene. This will lead to producing the hIL-12 gene fragment with NheI restriction sites after amplification by polymerase chain reaction (PCR) cycles. The PCR product was then purified by using MEGAquick-spinTM Total Fragment DNA Purification Kit. The purified hIL-12 was then cloned into pJET cloning vector by T4 DNA ligase and transformed into competent Escherichia. coli Top 10 cells by using heat-shock transformation method. The cloning was verified using NheI restriction enzyme digestion analysis and further confirmed by sequencing. 2015 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85123/1/FBSB%202015%20112%20-%20IR.pdf Che Ani, Muhamad Alhapis (2015) Cloning of human- interleukin-12 into pJET cloning vector for anti-cancer vaccine development. [Project Paper Report] |
| spellingShingle | Che Ani, Muhamad Alhapis Cloning of human- interleukin-12 into pJET cloning vector for anti-cancer vaccine development |
| title | Cloning of human- interleukin-12 into pJET cloning vector for anti-cancer vaccine development |
| title_full | Cloning of human- interleukin-12 into pJET cloning vector for anti-cancer vaccine development |
| title_fullStr | Cloning of human- interleukin-12 into pJET cloning vector for anti-cancer vaccine development |
| title_full_unstemmed | Cloning of human- interleukin-12 into pJET cloning vector for anti-cancer vaccine development |
| title_short | Cloning of human- interleukin-12 into pJET cloning vector for anti-cancer vaccine development |
| title_sort | cloning of human- interleukin-12 into pjet cloning vector for anti-cancer vaccine development |
| url | http://psasir.upm.edu.my/id/eprint/85123/ http://psasir.upm.edu.my/id/eprint/85123/1/FBSB%202015%20112%20-%20IR.pdf |