Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus
Protein A was produced extra cellularly by methicillinresistant Staphylococcus aureus, strain A676. A competitive- ELISA technique based on a competitive pinding between unlabelled protein A and conjugated protein A was employed to measure the concentration of protein A. A study was also conduct...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1994
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/8361/ http://psasir.upm.edu.my/id/eprint/8361/1/FSMB_1994_2_A.pdf |
| _version_ | 1848840848596271104 |
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| author | Tahir, Sharifah |
| author_facet | Tahir, Sharifah |
| author_sort | Tahir, Sharifah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Protein A was produced extra cellularly by methicillinresistant
Staphylococcus aureus, strain A676. A competitive-
ELISA technique based on a competitive pinding between unlabelled protein A and conjugated protein A was employed to
measure the concentration of protein A. A study was also
conducted to develop this technique. Rabbit IgG was first purified before it was used to coat a
microtiterplate. IgG purified using ammonium sulphate
precipitation in combination with DEAE-cellulose chromatography
gave purer IgG than the caprylic acid method combined with
saturated ammonium sulphate or single-step DEAE-cellulose ion
exchange chromatography. The molecular weight of the purified
IgG was 140,000 dalton based on SDS gelelectrophoresis. The
minimum detectable amount of protein A was in the range of 0-20
ng/ml. The optimum concentration of IgG required for coating
was 2pg/ml and the incubation time for the substrate was 20 minutes. |
| first_indexed | 2025-11-15T07:33:52Z |
| format | Thesis |
| id | upm-8361 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:33:52Z |
| publishDate | 1994 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-83612012-06-29T08:56:09Z http://psasir.upm.edu.my/id/eprint/8361/ Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus Tahir, Sharifah Protein A was produced extra cellularly by methicillinresistant Staphylococcus aureus, strain A676. A competitive- ELISA technique based on a competitive pinding between unlabelled protein A and conjugated protein A was employed to measure the concentration of protein A. A study was also conducted to develop this technique. Rabbit IgG was first purified before it was used to coat a microtiterplate. IgG purified using ammonium sulphate precipitation in combination with DEAE-cellulose chromatography gave purer IgG than the caprylic acid method combined with saturated ammonium sulphate or single-step DEAE-cellulose ion exchange chromatography. The molecular weight of the purified IgG was 140,000 dalton based on SDS gelelectrophoresis. The minimum detectable amount of protein A was in the range of 0-20 ng/ml. The optimum concentration of IgG required for coating was 2pg/ml and the incubation time for the substrate was 20 minutes. 1994 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8361/1/FSMB_1994_2_A.pdf Tahir, Sharifah (1994) Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus. Masters thesis, Universiti Pertanian Malaysia. Staphylococcus aureus. Proteins. Methicillin Resistance. English |
| spellingShingle | Staphylococcus aureus. Proteins. Methicillin Resistance. Tahir, Sharifah Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus |
| title | Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus |
| title_full | Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus |
| title_fullStr | Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus |
| title_full_unstemmed | Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus |
| title_short | Production of Extracellular Protein a from a Methicillin-Resistant Strain of Staphylococcus Aureus |
| title_sort | production of extracellular protein a from a methicillin-resistant strain of staphylococcus aureus |
| topic | Staphylococcus aureus. Proteins. Methicillin Resistance. |
| url | http://psasir.upm.edu.my/id/eprint/8361/ http://psasir.upm.edu.my/id/eprint/8361/1/FSMB_1994_2_A.pdf |