Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance...

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Main Authors: Arora, Sanu, Steuernagel, Burkhard, Gaurav, Kumar, Chandramohan, Sutha, Long, Yunming, Matny, Oadi, Johnson, Ryan, Enk, Jacob, Periyannan, Sambasivam, Singh, Narinder, Md Hatta, M. Asyraf, Athiyannan, Naveenkumar, Cheema, Jitender, Yu, Guotai, Kangara, Ngonidzashe, Ghosh, Sreya, Szabo, Les J., Poland, Jesse, Bariana, Harbans, Jones, Jonathan D. G., Bentley, Alison R., Ayliffe, Mick, Olson, Eric, Xu, Steven S., Steffenson, Brian J., Lagudah, Evans, Wulff, Brande B. H.
Format: Article
Language:English
Published: Nature Research 2019
Online Access:http://psasir.upm.edu.my/id/eprint/81994/
http://psasir.upm.edu.my/id/eprint/81994/1/Resistance%20gene%20cloning%20.pdf
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author Arora, Sanu
Steuernagel, Burkhard
Gaurav, Kumar
Chandramohan, Sutha
Long, Yunming
Matny, Oadi
Johnson, Ryan
Enk, Jacob
Periyannan, Sambasivam
Singh, Narinder
Md Hatta, M. Asyraf
Athiyannan, Naveenkumar
Cheema, Jitender
Yu, Guotai
Kangara, Ngonidzashe
Ghosh, Sreya
Szabo, Les J.
Poland, Jesse
Bariana, Harbans
Jones, Jonathan D. G.
Bentley, Alison R.
Ayliffe, Mick
Olson, Eric
Xu, Steven S.
Steffenson, Brian J.
Lagudah, Evans
Wulff, Brande B. H.
author_facet Arora, Sanu
Steuernagel, Burkhard
Gaurav, Kumar
Chandramohan, Sutha
Long, Yunming
Matny, Oadi
Johnson, Ryan
Enk, Jacob
Periyannan, Sambasivam
Singh, Narinder
Md Hatta, M. Asyraf
Athiyannan, Naveenkumar
Cheema, Jitender
Yu, Guotai
Kangara, Ngonidzashe
Ghosh, Sreya
Szabo, Les J.
Poland, Jesse
Bariana, Harbans
Jones, Jonathan D. G.
Bentley, Alison R.
Ayliffe, Mick
Olson, Eric
Xu, Steven S.
Steffenson, Brian J.
Lagudah, Evans
Wulff, Brande B. H.
author_sort Arora, Sanu
building UPM Institutional Repository
collection Online Access
description Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.
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institution Universiti Putra Malaysia
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language English
last_indexed 2025-11-15T12:25:47Z
publishDate 2019
publisher Nature Research
recordtype eprints
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spelling upm-819942021-09-08T02:44:36Z http://psasir.upm.edu.my/id/eprint/81994/ Resistance gene cloning from a wild crop relative by sequence capture and association genetics Arora, Sanu Steuernagel, Burkhard Gaurav, Kumar Chandramohan, Sutha Long, Yunming Matny, Oadi Johnson, Ryan Enk, Jacob Periyannan, Sambasivam Singh, Narinder Md Hatta, M. Asyraf Athiyannan, Naveenkumar Cheema, Jitender Yu, Guotai Kangara, Ngonidzashe Ghosh, Sreya Szabo, Les J. Poland, Jesse Bariana, Harbans Jones, Jonathan D. G. Bentley, Alison R. Ayliffe, Mick Olson, Eric Xu, Steven S. Steffenson, Brian J. Lagudah, Evans Wulff, Brande B. H. Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel. Nature Research 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81994/1/Resistance%20gene%20cloning%20.pdf Arora, Sanu and Steuernagel, Burkhard and Gaurav, Kumar and Chandramohan, Sutha and Long, Yunming and Matny, Oadi and Johnson, Ryan and Enk, Jacob and Periyannan, Sambasivam and Singh, Narinder and Md Hatta, M. Asyraf and Athiyannan, Naveenkumar and Cheema, Jitender and Yu, Guotai and Kangara, Ngonidzashe and Ghosh, Sreya and Szabo, Les J. and Poland, Jesse and Bariana, Harbans and Jones, Jonathan D. G. and Bentley, Alison R. and Ayliffe, Mick and Olson, Eric and Xu, Steven S. and Steffenson, Brian J. and Lagudah, Evans and Wulff, Brande B. H. (2019) Resistance gene cloning from a wild crop relative by sequence capture and association genetics. Nature Biotechnology, 37 (2). pp. 139-143. ISSN 1087-0156; ESSN: 1546-1696 https://www.nature.com/articles/s41587-018-0007-9 10.1038/s41587-018-0007-9
spellingShingle Arora, Sanu
Steuernagel, Burkhard
Gaurav, Kumar
Chandramohan, Sutha
Long, Yunming
Matny, Oadi
Johnson, Ryan
Enk, Jacob
Periyannan, Sambasivam
Singh, Narinder
Md Hatta, M. Asyraf
Athiyannan, Naveenkumar
Cheema, Jitender
Yu, Guotai
Kangara, Ngonidzashe
Ghosh, Sreya
Szabo, Les J.
Poland, Jesse
Bariana, Harbans
Jones, Jonathan D. G.
Bentley, Alison R.
Ayliffe, Mick
Olson, Eric
Xu, Steven S.
Steffenson, Brian J.
Lagudah, Evans
Wulff, Brande B. H.
Resistance gene cloning from a wild crop relative by sequence capture and association genetics
title Resistance gene cloning from a wild crop relative by sequence capture and association genetics
title_full Resistance gene cloning from a wild crop relative by sequence capture and association genetics
title_fullStr Resistance gene cloning from a wild crop relative by sequence capture and association genetics
title_full_unstemmed Resistance gene cloning from a wild crop relative by sequence capture and association genetics
title_short Resistance gene cloning from a wild crop relative by sequence capture and association genetics
title_sort resistance gene cloning from a wild crop relative by sequence capture and association genetics
url http://psasir.upm.edu.my/id/eprint/81994/
http://psasir.upm.edu.my/id/eprint/81994/
http://psasir.upm.edu.my/id/eprint/81994/
http://psasir.upm.edu.my/id/eprint/81994/1/Resistance%20gene%20cloning%20.pdf