Microbial study of pH sensitive starch based film using agar diffusion method (zone inhibition assay)
Active and smart packaging is a promising form of food packaging that offers a great economical potential due to consumer demand for a packaging that accommodate a hectic way of life. An antimicrobial film with pH colour indicator (pHF) can be made by incorporating suitable antimicrobial (AM) agent...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81351/ http://psasir.upm.edu.my/id/eprint/81351/1/Microbial%20study%20of%20pH%20sensitive%20starch%20based%20film%20using%20agar%20diffusion%20method%20%28zone%20inhibition%20assay%29.pdf |
| _version_ | 1848859082619879424 |
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| author | Khairuddin, Nozieana Muhamad, Ida Idayu Wan Abdul Rahman, Wan Aizan Siddique, Bazlul Mobin |
| author_facet | Khairuddin, Nozieana Muhamad, Ida Idayu Wan Abdul Rahman, Wan Aizan Siddique, Bazlul Mobin |
| author_sort | Khairuddin, Nozieana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Active and smart packaging is a promising form of food packaging that offers a great economical potential due to consumer demand for a packaging that accommodate a hectic way of life. An antimicrobial film with pH colour indicator (pHF) can be made by incorporating suitable antimicrobial (AM) agent and colour indicator into food package matrices whilst applying a bio switch concept to inhibit the pathogenic microorganisms and respond automatically to changes (external stimuli) in the environment. The present work aimed to study the developed formulation of hydroxyethylcellulose (HEC)/wheat-starch based pHF film in which the active compound, thymol (0.5, 1, 1.5, 2, and 2.5% w/w) and 50:50% w/w bromothymol blue and methyl red (as the colour indicator) against microbial growth. A solution casting method was used in the film preparation while thymol and colourant were incorporated prior to casting. The effect of thymol showed a range of microbial inhibition zones of 16.3 - 26.4% and 22.1 - 39.9% towards E. coli and B. subtilis, respectively. Whilst, a lower inhibition zone of 0.4 - 5.1% was demonstrated for fungus A. niger. |
| first_indexed | 2025-11-15T12:23:41Z |
| format | Article |
| id | upm-81351 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:23:41Z |
| publishDate | 2019 |
| publisher | IOP Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-813512021-02-22T20:28:39Z http://psasir.upm.edu.my/id/eprint/81351/ Microbial study of pH sensitive starch based film using agar diffusion method (zone inhibition assay) Khairuddin, Nozieana Muhamad, Ida Idayu Wan Abdul Rahman, Wan Aizan Siddique, Bazlul Mobin Active and smart packaging is a promising form of food packaging that offers a great economical potential due to consumer demand for a packaging that accommodate a hectic way of life. An antimicrobial film with pH colour indicator (pHF) can be made by incorporating suitable antimicrobial (AM) agent and colour indicator into food package matrices whilst applying a bio switch concept to inhibit the pathogenic microorganisms and respond automatically to changes (external stimuli) in the environment. The present work aimed to study the developed formulation of hydroxyethylcellulose (HEC)/wheat-starch based pHF film in which the active compound, thymol (0.5, 1, 1.5, 2, and 2.5% w/w) and 50:50% w/w bromothymol blue and methyl red (as the colour indicator) against microbial growth. A solution casting method was used in the film preparation while thymol and colourant were incorporated prior to casting. The effect of thymol showed a range of microbial inhibition zones of 16.3 - 26.4% and 22.1 - 39.9% towards E. coli and B. subtilis, respectively. Whilst, a lower inhibition zone of 0.4 - 5.1% was demonstrated for fungus A. niger. IOP Publishing 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81351/1/Microbial%20study%20of%20pH%20sensitive%20starch%20based%20film%20using%20agar%20diffusion%20method%20%28zone%20inhibition%20assay%29.pdf Khairuddin, Nozieana and Muhamad, Ida Idayu and Wan Abdul Rahman, Wan Aizan and Siddique, Bazlul Mobin (2019) Microbial study of pH sensitive starch based film using agar diffusion method (zone inhibition assay). IOP Conference Series: Materials Science and Engineering, 607. art. no. 012007. pp. 1-6. ISSN 1757-8981; ESSN: 1757-899X https://iopscience.iop.org/article/10.1088/1757-899X/607/1/012007 10.1088/1757-899X/607/1/012007 |
| spellingShingle | Khairuddin, Nozieana Muhamad, Ida Idayu Wan Abdul Rahman, Wan Aizan Siddique, Bazlul Mobin Microbial study of pH sensitive starch based film using agar diffusion method (zone inhibition assay) |
| title | Microbial study of pH sensitive starch based film using agar diffusion method (zone inhibition assay) |
| title_full | Microbial study of pH sensitive starch based film using agar diffusion method (zone inhibition assay) |
| title_fullStr | Microbial study of pH sensitive starch based film using agar diffusion method (zone inhibition assay) |
| title_full_unstemmed | Microbial study of pH sensitive starch based film using agar diffusion method (zone inhibition assay) |
| title_short | Microbial study of pH sensitive starch based film using agar diffusion method (zone inhibition assay) |
| title_sort | microbial study of ph sensitive starch based film using agar diffusion method (zone inhibition assay) |
| url | http://psasir.upm.edu.my/id/eprint/81351/ http://psasir.upm.edu.my/id/eprint/81351/ http://psasir.upm.edu.my/id/eprint/81351/ http://psasir.upm.edu.my/id/eprint/81351/1/Microbial%20study%20of%20pH%20sensitive%20starch%20based%20film%20using%20agar%20diffusion%20method%20%28zone%20inhibition%20assay%29.pdf |