Meat molecular detection: sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin
Three restriction enzymes were used in Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using the mitochondrial cytochrome b region to establish a differential diagnosis which detect and discriminate between three meat species: pork, cow and chicken. DNA was extracted f...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Putra Malaysia Press
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/800/ http://psasir.upm.edu.my/id/eprint/800/1/51-59.pdf |
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| author | Ong, Sang Bing Mat Issa @ Zakaria, Zuraini Gunsalam, Jurin Wolmon Cheah, Yoke Kqueen Robin, Tunung Chai, Lay Ching Yuli, Haryani Mohamad Ghazali, Farinazleen Radu, Son |
| author_facet | Ong, Sang Bing Mat Issa @ Zakaria, Zuraini Gunsalam, Jurin Wolmon Cheah, Yoke Kqueen Robin, Tunung Chai, Lay Ching Yuli, Haryani Mohamad Ghazali, Farinazleen Radu, Son |
| author_sort | Ong, Sang Bing |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Three restriction enzymes were used in Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using the mitochondrial cytochrome b region to establish a differential diagnosis which detect and discriminate between three meat species: pork, cow and
chicken. DNA was extracted from samples containing meat of a single animal such as raw pork (Sus scrofa domesticus), chicken (Gallus gallus) and cow (Bos taurus) as well as mixed samples of two species of animals in different ratios. The amplified 359 base pairs (bp) portion of the
mitochondrial cyt b gene from pure or mixed samples in different ratios was cut using three different restriction enzymes resulting in species specific restriction fragment length polymorphism (RFLP). This technique proved to be extremely reliable in detecting the presence of low levels of target DNA obtained from a 0.25 mg component in a particular mixed meat sample. This revealed the cyt b region as highly conserved and consequently a good molecular
marker for diagnostic studies. Thus, this technique can be applied to food authentication for the identification of different species of animals in food products. |
| first_indexed | 2025-11-15T07:02:13Z |
| format | Article |
| id | upm-800 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:02:13Z |
| publishDate | 2007 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-8002016-11-15T03:47:31Z http://psasir.upm.edu.my/id/eprint/800/ Meat molecular detection: sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin Ong, Sang Bing Mat Issa @ Zakaria, Zuraini Gunsalam, Jurin Wolmon Cheah, Yoke Kqueen Robin, Tunung Chai, Lay Ching Yuli, Haryani Mohamad Ghazali, Farinazleen Radu, Son Three restriction enzymes were used in Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using the mitochondrial cytochrome b region to establish a differential diagnosis which detect and discriminate between three meat species: pork, cow and chicken. DNA was extracted from samples containing meat of a single animal such as raw pork (Sus scrofa domesticus), chicken (Gallus gallus) and cow (Bos taurus) as well as mixed samples of two species of animals in different ratios. The amplified 359 base pairs (bp) portion of the mitochondrial cyt b gene from pure or mixed samples in different ratios was cut using three different restriction enzymes resulting in species specific restriction fragment length polymorphism (RFLP). This technique proved to be extremely reliable in detecting the presence of low levels of target DNA obtained from a 0.25 mg component in a particular mixed meat sample. This revealed the cyt b region as highly conserved and consequently a good molecular marker for diagnostic studies. Thus, this technique can be applied to food authentication for the identification of different species of animals in food products. Universiti Putra Malaysia Press 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/800/1/51-59.pdf Ong, Sang Bing and Mat Issa @ Zakaria, Zuraini and Gunsalam, Jurin Wolmon and Cheah, Yoke Kqueen and Robin, Tunung and Chai, Lay Ching and Yuli, Haryani and Mohamad Ghazali, Farinazleen and Radu, Son (2007) Meat molecular detection: sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin. ASEAN Food Journal, 14 (1). pp. 51-59. ISSN 0127-7324; ESSN: 1505-5337 http://www.ifrj.upm.edu.my/afjv14%281%292007/51-59.pdf |
| spellingShingle | Ong, Sang Bing Mat Issa @ Zakaria, Zuraini Gunsalam, Jurin Wolmon Cheah, Yoke Kqueen Robin, Tunung Chai, Lay Ching Yuli, Haryani Mohamad Ghazali, Farinazleen Radu, Son Meat molecular detection: sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin |
| title | Meat molecular detection: sensitivity of polymerase chain
reaction-restriction fragment length polymorphism in species
differentiation of meat from animal origin |
| title_full | Meat molecular detection: sensitivity of polymerase chain
reaction-restriction fragment length polymorphism in species
differentiation of meat from animal origin |
| title_fullStr | Meat molecular detection: sensitivity of polymerase chain
reaction-restriction fragment length polymorphism in species
differentiation of meat from animal origin |
| title_full_unstemmed | Meat molecular detection: sensitivity of polymerase chain
reaction-restriction fragment length polymorphism in species
differentiation of meat from animal origin |
| title_short | Meat molecular detection: sensitivity of polymerase chain
reaction-restriction fragment length polymorphism in species
differentiation of meat from animal origin |
| title_sort | meat molecular detection: sensitivity of polymerase chain
reaction-restriction fragment length polymorphism in species
differentiation of meat from animal origin |
| url | http://psasir.upm.edu.my/id/eprint/800/ http://psasir.upm.edu.my/id/eprint/800/ http://psasir.upm.edu.my/id/eprint/800/1/51-59.pdf |