Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken
An accurate and sensitive liquid chromatography-electrospray ionisation/multi stage mass spectrometry (LC–ESI–MS/MS) method has been developed for the characterization and quantitation of nine heterocyclic amine (HCA) compounds. We were identified 2-Amino-3-methyl-3H-imidazo[4,5-F]quinoline (IQ), 2-...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79480/ http://psasir.upm.edu.my/id/eprint/79480/1/Effect%20of%20a%20different%20mobile%20phase%20.pdf |
| _version_ | 1848858690420998144 |
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| author | Selamat, Jinap Jaafar, Siham N. Hasnol, Nur Diyana Syamim Jahurul, M. H. A. |
| author_facet | Selamat, Jinap Jaafar, Siham N. Hasnol, Nur Diyana Syamim Jahurul, M. H. A. |
| author_sort | Selamat, Jinap |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An accurate and sensitive liquid chromatography-electrospray ionisation/multi stage mass spectrometry (LC–ESI–MS/MS) method has been developed for the characterization and quantitation of nine heterocyclic amine (HCA) compounds. We were identified 2-Amino-3-methyl-3H-imidazo[4,5-F]quinoline (IQ), 2-Amino-3-methyl-3H-imidazo[4,5-f]quinoxaline (IQx), 2-Amino-3,4-dimethyl-3H-imidazo[4,5-f]quinoline (MeIQ), 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-Amino-3,7,8-trimethyl-3H-imidazo[4,5-f]quinoxaline (7,8-DiMeIQx), 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine as polar molecules, and they were eluted accordingly at the beginning of the analysis. However, Norharman, Harman and AαC were identified as a non-polar molecules and were eluted last. The excellent selectivity and sensitivity achieved by the selected reaction monitoring mode permitted satisfactory quantitation and confirmation of the injected HCA compounds with limit of detection and quantitation ranges from 0.17 to 1.44 pg and 0.53 to 4.57 pg, respectively. The recoveries ranged from 74 to 108%, with an interday and intraday precision of 1.6–1.9% and 0.1–0.9%, respectively. The method was successfully applied to identify and quantify the studied HCAs in fried and grilled chicken. |
| first_indexed | 2025-11-15T12:17:27Z |
| format | Article |
| id | upm-79480 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:17:27Z |
| publishDate | 2020 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-794802021-05-26T22:58:35Z http://psasir.upm.edu.my/id/eprint/79480/ Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken Selamat, Jinap Jaafar, Siham N. Hasnol, Nur Diyana Syamim Jahurul, M. H. A. An accurate and sensitive liquid chromatography-electrospray ionisation/multi stage mass spectrometry (LC–ESI–MS/MS) method has been developed for the characterization and quantitation of nine heterocyclic amine (HCA) compounds. We were identified 2-Amino-3-methyl-3H-imidazo[4,5-F]quinoline (IQ), 2-Amino-3-methyl-3H-imidazo[4,5-f]quinoxaline (IQx), 2-Amino-3,4-dimethyl-3H-imidazo[4,5-f]quinoline (MeIQ), 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-Amino-3,7,8-trimethyl-3H-imidazo[4,5-f]quinoxaline (7,8-DiMeIQx), 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine as polar molecules, and they were eluted accordingly at the beginning of the analysis. However, Norharman, Harman and AαC were identified as a non-polar molecules and were eluted last. The excellent selectivity and sensitivity achieved by the selected reaction monitoring mode permitted satisfactory quantitation and confirmation of the injected HCA compounds with limit of detection and quantitation ranges from 0.17 to 1.44 pg and 0.53 to 4.57 pg, respectively. The recoveries ranged from 74 to 108%, with an interday and intraday precision of 1.6–1.9% and 0.1–0.9%, respectively. The method was successfully applied to identify and quantify the studied HCAs in fried and grilled chicken. Springer 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/79480/1/Effect%20of%20a%20different%20mobile%20phase%20.pdf Selamat, Jinap and Jaafar, Siham N. and Hasnol, Nur Diyana Syamim and Jahurul, M. H. A. (2020) Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken. Journal of Food Measurement and Characterization, 14. pp. 262-271. ISSN 2193-4126; ESSN: 2193-4134 https://link.springer.com/article/10.1007/s11694-019-00288-0 10.1007/s11694-019-00288-0 |
| spellingShingle | Selamat, Jinap Jaafar, Siham N. Hasnol, Nur Diyana Syamim Jahurul, M. H. A. Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_full | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_fullStr | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_full_unstemmed | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_short | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_sort | effect of a different mobile phase on lc–esi–ms/ms performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| url | http://psasir.upm.edu.my/id/eprint/79480/ http://psasir.upm.edu.my/id/eprint/79480/ http://psasir.upm.edu.my/id/eprint/79480/ http://psasir.upm.edu.my/id/eprint/79480/1/Effect%20of%20a%20different%20mobile%20phase%20.pdf |