The effects of methanol extracted Areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats

The extensive use of chemical anthelmintics in parasitic gastroenteritis (PGE) management has created resistance, thus reducing their effectiveness. The present study was conducted to compare the effectiveness of Areca catechu (betel nut) extract and levamisole on survival rate of the third st...

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Main Author: Mat Desa, Nurul Farliana
Format: Project Paper Report
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/78189/
http://psasir.upm.edu.my/id/eprint/78189/1/FPV%202016%2039%20-%20IR.pdf
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author Mat Desa, Nurul Farliana
author_facet Mat Desa, Nurul Farliana
author_sort Mat Desa, Nurul Farliana
building UPM Institutional Repository
collection Online Access
description The extensive use of chemical anthelmintics in parasitic gastroenteritis (PGE) management has created resistance, thus reducing their effectiveness. The present study was conducted to compare the effectiveness of Areca catechu (betel nut) extract and levamisole on survival rate of the third stage larvae (L3) strongyles of goat and to determine effective concentration of betel nut extract (BNE) for larvacidal activity against of L3 strongyles in in-vitro condition. Fresh fecal materials of naturally infected goats were collected and the second stage larvae were harvested after 7 days to estimate their numbers. Methanolic extract of betel nut at 300, 600 and 1200 µg/ml, Levamisole at 10 mg/ml and PBS plus 0.1% DMSO were used to determine their larvacidal effects. BNE at 1200 µg/ml showed 61.70% of L3 died within 24-hours post exposure while Levamisole at 10 mg/ml showed 100% mortality rate of L3 within 2-hours post exposure (p<0.05). The highest L3 strongyles death percentage (43.40%) belongs to Oesophagostomum sp, followed by 40.90% of Haemonchus spand 15.70% of Trichostrongylus sp. Although the effects of BNE is not as effective as levamisole, it has a considerable anthelmintic activity against strongyles larvae in goats and can be used as an alternative anthelmintic in future.
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institution Universiti Putra Malaysia
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spelling upm-781892025-01-03T07:49:24Z http://psasir.upm.edu.my/id/eprint/78189/ The effects of methanol extracted Areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats Mat Desa, Nurul Farliana The extensive use of chemical anthelmintics in parasitic gastroenteritis (PGE) management has created resistance, thus reducing their effectiveness. The present study was conducted to compare the effectiveness of Areca catechu (betel nut) extract and levamisole on survival rate of the third stage larvae (L3) strongyles of goat and to determine effective concentration of betel nut extract (BNE) for larvacidal activity against of L3 strongyles in in-vitro condition. Fresh fecal materials of naturally infected goats were collected and the second stage larvae were harvested after 7 days to estimate their numbers. Methanolic extract of betel nut at 300, 600 and 1200 µg/ml, Levamisole at 10 mg/ml and PBS plus 0.1% DMSO were used to determine their larvacidal effects. BNE at 1200 µg/ml showed 61.70% of L3 died within 24-hours post exposure while Levamisole at 10 mg/ml showed 100% mortality rate of L3 within 2-hours post exposure (p<0.05). The highest L3 strongyles death percentage (43.40%) belongs to Oesophagostomum sp, followed by 40.90% of Haemonchus spand 15.70% of Trichostrongylus sp. Although the effects of BNE is not as effective as levamisole, it has a considerable anthelmintic activity against strongyles larvae in goats and can be used as an alternative anthelmintic in future. 2016-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/78189/1/FPV%202016%2039%20-%20IR.pdf Mat Desa, Nurul Farliana (2016) The effects of methanol extracted Areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats. [Project Paper Report]
spellingShingle Mat Desa, Nurul Farliana
The effects of methanol extracted Areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats
title The effects of methanol extracted Areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats
title_full The effects of methanol extracted Areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats
title_fullStr The effects of methanol extracted Areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats
title_full_unstemmed The effects of methanol extracted Areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats
title_short The effects of methanol extracted Areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats
title_sort effects of methanol extracted areca catechu (betel nut) and levamisole on in-vitro survival rate of strongyles in goats
url http://psasir.upm.edu.my/id/eprint/78189/
http://psasir.upm.edu.my/id/eprint/78189/1/FPV%202016%2039%20-%20IR.pdf