Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma
A sensitive and accurate high performance liquid chromatography with ultraviolet/visible light detection (HPLC-UV/VIS) method for the quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma was developed and validated. BHMC and the internal standard, harmaline,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/78036/ http://psasir.upm.edu.my/id/eprint/78036/1/78036.pdf |
| _version_ | 1848858395276214272 |
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| author | Lee, Yu Zhao Lee, Ming Tatt Lajis, Nordin Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad Tham, Chau Ling |
| author_facet | Lee, Yu Zhao Lee, Ming Tatt Lajis, Nordin Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad Tham, Chau Ling |
| author_sort | Lee, Yu Zhao |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A sensitive and accurate high performance liquid chromatography with ultraviolet/visible light detection (HPLC-UV/VIS) method for the quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma was developed and validated. BHMC and the internal standard, harmaline, were extracted from plasma samples by a simple liquid–liquid extraction using 95% ethyl acetate and 5% methanol. Plasma concentration of BHMC and internal standard were analyzed by reversed phase chromatography using a C18 column (150 × 4.6 mm I.D., particle size 5 µm) and elution with a gradient mobile phase of water and methanol at a flow rate of 1.0 mL/min. Detection of BHMC and internal standard was done at a wavelength of 380 nm. The limit of quantification was 0.02 µg/mL. The calibration curves was linear (R2 > 0.999) over the concentration range of 0.02–2.5 µg/mL. Intra- and inter-day precision were less than 2% coefficient of variation. The validated method was then applied to a pharmacokinetic study in rats by intravenous administration of BHMC at a single dose of 10 mg/kg. Pharmacokinetic parameters such as half-life, maximum plasma concentration, volume of distribution, clearance and elimination rate constant for BHMC were calculated. |
| first_indexed | 2025-11-15T12:12:46Z |
| format | Article |
| id | upm-78036 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:12:46Z |
| publishDate | 2012 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-780362020-05-03T22:32:55Z http://psasir.upm.edu.my/id/eprint/78036/ Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma Lee, Yu Zhao Lee, Ming Tatt Lajis, Nordin Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad Tham, Chau Ling A sensitive and accurate high performance liquid chromatography with ultraviolet/visible light detection (HPLC-UV/VIS) method for the quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma was developed and validated. BHMC and the internal standard, harmaline, were extracted from plasma samples by a simple liquid–liquid extraction using 95% ethyl acetate and 5% methanol. Plasma concentration of BHMC and internal standard were analyzed by reversed phase chromatography using a C18 column (150 × 4.6 mm I.D., particle size 5 µm) and elution with a gradient mobile phase of water and methanol at a flow rate of 1.0 mL/min. Detection of BHMC and internal standard was done at a wavelength of 380 nm. The limit of quantification was 0.02 µg/mL. The calibration curves was linear (R2 > 0.999) over the concentration range of 0.02–2.5 µg/mL. Intra- and inter-day precision were less than 2% coefficient of variation. The validated method was then applied to a pharmacokinetic study in rats by intravenous administration of BHMC at a single dose of 10 mg/kg. Pharmacokinetic parameters such as half-life, maximum plasma concentration, volume of distribution, clearance and elimination rate constant for BHMC were calculated. MDPI 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/78036/1/78036.pdf Lee, Yu Zhao and Lee, Ming Tatt and Lajis, Nordin and Sulaiman, Mohd Roslan and Israf Ali, Daud Ahmad and Tham, Chau Ling (2012) Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma. Molecules, 17 (12). pp. 14555-14564. ISSN 1420-3049 https://www.mdpi.com/1420-3049/17/12/14555 10.3390/molecules171214555 |
| spellingShingle | Lee, Yu Zhao Lee, Ming Tatt Lajis, Nordin Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad Tham, Chau Ling Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma |
| title | Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma |
| title_full | Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma |
| title_fullStr | Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma |
| title_full_unstemmed | Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma |
| title_short | Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma |
| title_sort | development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (bhmc) in rat plasma |
| url | http://psasir.upm.edu.my/id/eprint/78036/ http://psasir.upm.edu.my/id/eprint/78036/ http://psasir.upm.edu.my/id/eprint/78036/ http://psasir.upm.edu.my/id/eprint/78036/1/78036.pdf |