Antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction

Solid phase extraction (SPE) using Sep-Pak® cartridges is one of the techniques used for fractionation of antioxidant compounds in waste of dabai oil extraction (defatted dabai parts). The aim of this study was to determine the phenolic compounds and antioxidant capacity in crude extracts and severa...

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Main Authors: Khoo, Hock Eng, Azlan, Azrina, Ismail, Amin, Abas, Faridah
Format: Article
Language:English
Published: MDPI 2012
Online Access:http://psasir.upm.edu.my/id/eprint/78010/
http://psasir.upm.edu.my/id/eprint/78010/1/78010.pdf
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author Khoo, Hock Eng
Azlan, Azrina
Ismail, Amin
Abas, Faridah
author_facet Khoo, Hock Eng
Azlan, Azrina
Ismail, Amin
Abas, Faridah
author_sort Khoo, Hock Eng
building UPM Institutional Repository
collection Online Access
description Solid phase extraction (SPE) using Sep-Pak® cartridges is one of the techniques used for fractionation of antioxidant compounds in waste of dabai oil extraction (defatted dabai parts). The aim of this study was to determine the phenolic compounds and antioxidant capacity in crude extracts and several SPE fractions from methanolic extract of defatted dabai pulp and peel. Based on SPE, Sep-Pak® cyanopropyl and C18 cartridges were used to fractionate the antioxidant-rich crude extracts into water and methanolic fractions. Analyzed using LC-MS, flavonoids, anthocyanins, saponin derivatives and other unknown antioxidative compounds were detected in the defatted dabai crude extracts and their SPE fractions. Anthocyanins were the major phenolic compounds identified in the defatted dabai peel and detected in most of the SPE fractions. Methanolic fractions of defatted dabai parts embraced higher total phenolics and antioxidant capacity than water fractions. This finding also revealed the crude extracts of defatted dabai peel have the most significant antioxidant properties compared to the methanolic and water fractions studied. The crude extract of defatted dabai parts remain as the most potent antioxidant as it contains mixture of flavonoids, anthocyanins and other potential antioxidants.
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spelling upm-780102020-06-01T09:04:43Z http://psasir.upm.edu.my/id/eprint/78010/ Antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction Khoo, Hock Eng Azlan, Azrina Ismail, Amin Abas, Faridah Solid phase extraction (SPE) using Sep-Pak® cartridges is one of the techniques used for fractionation of antioxidant compounds in waste of dabai oil extraction (defatted dabai parts). The aim of this study was to determine the phenolic compounds and antioxidant capacity in crude extracts and several SPE fractions from methanolic extract of defatted dabai pulp and peel. Based on SPE, Sep-Pak® cyanopropyl and C18 cartridges were used to fractionate the antioxidant-rich crude extracts into water and methanolic fractions. Analyzed using LC-MS, flavonoids, anthocyanins, saponin derivatives and other unknown antioxidative compounds were detected in the defatted dabai crude extracts and their SPE fractions. Anthocyanins were the major phenolic compounds identified in the defatted dabai peel and detected in most of the SPE fractions. Methanolic fractions of defatted dabai parts embraced higher total phenolics and antioxidant capacity than water fractions. This finding also revealed the crude extracts of defatted dabai peel have the most significant antioxidant properties compared to the methanolic and water fractions studied. The crude extract of defatted dabai parts remain as the most potent antioxidant as it contains mixture of flavonoids, anthocyanins and other potential antioxidants. MDPI 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/78010/1/78010.pdf Khoo, Hock Eng and Azlan, Azrina and Ismail, Amin and Abas, Faridah (2012) Antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction. Molecules, 17 (8). pp. 9754-9773. ISSN 1420-3049 https://www.mdpi.com/1420-3049/17/8/9754 10.3390/molecules17089754
spellingShingle Khoo, Hock Eng
Azlan, Azrina
Ismail, Amin
Abas, Faridah
Antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction
title Antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction
title_full Antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction
title_fullStr Antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction
title_full_unstemmed Antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction
title_short Antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction
title_sort antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction
url http://psasir.upm.edu.my/id/eprint/78010/
http://psasir.upm.edu.my/id/eprint/78010/
http://psasir.upm.edu.my/id/eprint/78010/
http://psasir.upm.edu.my/id/eprint/78010/1/78010.pdf