Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography
This work presents a simple procedure for pre-column derivatization of glyphosate and aminomethylphosphonic acid (AMPA) and their determination by high-performance liquid chromatography (HPLC). Derivatization was achieved by mixing a solution of 0.02 M FMOC-Cl, 0.05 M borate buffer and glyphosate or...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Yildiz Technical University
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/73893/ http://psasir.upm.edu.my/id/eprint/73893/1/Simplified%20method%20for%20derivatization.pdf |
| _version_ | 1848857389484212224 |
|---|---|
| author | Garba, Jamilu Samsuri, Abd Wahid Othman, Radziah Ahmad Hamdani, Muhammad Saiful |
| author_facet | Garba, Jamilu Samsuri, Abd Wahid Othman, Radziah Ahmad Hamdani, Muhammad Saiful |
| author_sort | Garba, Jamilu |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This work presents a simple procedure for pre-column derivatization of glyphosate and aminomethylphosphonic acid (AMPA) and their determination by high-performance liquid chromatography (HPLC). Derivatization was achieved by mixing a solution of 0.02 M FMOC-Cl, 0.05 M borate buffer and glyphosate or AMPA, then shaken for 1 hour, later washed with diethyl ether and ready for analysis. The quantification was performed by HPLC with fluorescent (FLD) or diode array detector (DAD). The result of the HPLC-FLD/DAD showed high linearity (R2 ≥ 0.995) of both compounds over eight point’s concentration range and their high recovery from water compared to soil matrixes. The relative standard deviation (RSD) range from 0.1 to 30 % from the aforementioned matrixes. The limit of detection of HPLC-FLD for glyphosate from water, sandy and clay soil was 0.008 mg L-1, 0.021 and 0.132 mg kg-1 respectively while that of AMPA was 0.004 mg L-1, 0.74 and 0.224 mg kg-1. Meanwhile, the limit of detection of HPLC-DAD for glyphosate from water, sandy and clay soils was 0.024 mg L-1, 0.731 and 0.122 mg kg-1 respectively while that of AMPA for water sample was 0.076 mg L-1. This study was unable to determine lower detection limit for AMPA from soil matrixes by HPLC-DAD thus suggested for more repeated extraction for increasing quantification of the compound. |
| first_indexed | 2025-11-15T11:56:46Z |
| format | Article |
| id | upm-73893 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:56:46Z |
| publishDate | 2018 |
| publisher | Yildiz Technical University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-738932022-05-17T08:45:52Z http://psasir.upm.edu.my/id/eprint/73893/ Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography Garba, Jamilu Samsuri, Abd Wahid Othman, Radziah Ahmad Hamdani, Muhammad Saiful This work presents a simple procedure for pre-column derivatization of glyphosate and aminomethylphosphonic acid (AMPA) and their determination by high-performance liquid chromatography (HPLC). Derivatization was achieved by mixing a solution of 0.02 M FMOC-Cl, 0.05 M borate buffer and glyphosate or AMPA, then shaken for 1 hour, later washed with diethyl ether and ready for analysis. The quantification was performed by HPLC with fluorescent (FLD) or diode array detector (DAD). The result of the HPLC-FLD/DAD showed high linearity (R2 ≥ 0.995) of both compounds over eight point’s concentration range and their high recovery from water compared to soil matrixes. The relative standard deviation (RSD) range from 0.1 to 30 % from the aforementioned matrixes. The limit of detection of HPLC-FLD for glyphosate from water, sandy and clay soil was 0.008 mg L-1, 0.021 and 0.132 mg kg-1 respectively while that of AMPA was 0.004 mg L-1, 0.74 and 0.224 mg kg-1. Meanwhile, the limit of detection of HPLC-DAD for glyphosate from water, sandy and clay soils was 0.024 mg L-1, 0.731 and 0.122 mg kg-1 respectively while that of AMPA for water sample was 0.076 mg L-1. This study was unable to determine lower detection limit for AMPA from soil matrixes by HPLC-DAD thus suggested for more repeated extraction for increasing quantification of the compound. Yildiz Technical University 2018-04-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73893/1/Simplified%20method%20for%20derivatization.pdf Garba, Jamilu and Samsuri, Abd Wahid and Othman, Radziah and Ahmad Hamdani, Muhammad Saiful (2018) Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography. Environmental Research & Technology, 1 (2). 19 - 30. ISSN 2636-8498 https://dergipark.org.tr/en/pub/ert/issue/36278/404155 |
| spellingShingle | Garba, Jamilu Samsuri, Abd Wahid Othman, Radziah Ahmad Hamdani, Muhammad Saiful Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography |
| title | Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography |
| title_full | Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography |
| title_fullStr | Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography |
| title_full_unstemmed | Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography |
| title_short | Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography |
| title_sort | simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography |
| url | http://psasir.upm.edu.my/id/eprint/73893/ http://psasir.upm.edu.my/id/eprint/73893/ http://psasir.upm.edu.my/id/eprint/73893/1/Simplified%20method%20for%20derivatization.pdf |