Shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge
The microbiological effects of palm oil mill effluent (POME) final discharge upon a receiving river were assessed in this study by using the nucleic acid double staining assay based on flow cytometry. The functional status of the bacterial community at the single-cell level was determined with regar...
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73883/ http://psasir.upm.edu.my/id/eprint/73883/1/PALM.pdf |
| _version_ | 1848857387279056896 |
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| author | Sharuddin, Siti Suhailah Ramli, Norhayati Mohd Nor, Diana Hassan, Mohd Ali Maeda, Toshinari Shirai, Yoshihito Sakai, Kenji Tashiro, Yukihiro |
| author_facet | Sharuddin, Siti Suhailah Ramli, Norhayati Mohd Nor, Diana Hassan, Mohd Ali Maeda, Toshinari Shirai, Yoshihito Sakai, Kenji Tashiro, Yukihiro |
| author_sort | Sharuddin, Siti Suhailah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The microbiological effects of palm oil mill effluent (POME) final discharge upon a receiving river were assessed in this study by using the nucleic acid double staining assay based on flow cytometry. The functional status of the bacterial community at the single-cell level was determined with regards to their abundance, viability and nucleic acid content to monitor the effects of POME final discharge on the affected river. The effluent resulted in the increment of the total cell concentration (TCC) and viable cells which were correlated with the increment of biological oxygen demand (BOD5) and total organic carbon (TOC) concentrations in the receiving river. The shift of low nucleic acid (LNA) to high nucleic acid (HNA) bacterial cells in the affected river suggested the transformation of dormant to active cells due to the POME final discharge. This is the first study to report on the shift of LNA/HNA ratios which may serves as a potential bioindicator in the screening of the anthropogenic effects due to POME final discharge in river water with originally high LNA proportions. Monitoring the effluent discharge at low trophic level using flow cytometry is a rapid and sensitive approach when compared to the current physicochemical assessment method. This approach allows for the screening of river water contamination caused by POME final discharge prior to a full assessment using the recently proposed specific bacterial indicators. |
| first_indexed | 2025-11-15T11:56:44Z |
| format | Article |
| id | upm-73883 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:56:44Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-738832021-06-21T11:16:58Z http://psasir.upm.edu.my/id/eprint/73883/ Shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge Sharuddin, Siti Suhailah Ramli, Norhayati Mohd Nor, Diana Hassan, Mohd Ali Maeda, Toshinari Shirai, Yoshihito Sakai, Kenji Tashiro, Yukihiro The microbiological effects of palm oil mill effluent (POME) final discharge upon a receiving river were assessed in this study by using the nucleic acid double staining assay based on flow cytometry. The functional status of the bacterial community at the single-cell level was determined with regards to their abundance, viability and nucleic acid content to monitor the effects of POME final discharge on the affected river. The effluent resulted in the increment of the total cell concentration (TCC) and viable cells which were correlated with the increment of biological oxygen demand (BOD5) and total organic carbon (TOC) concentrations in the receiving river. The shift of low nucleic acid (LNA) to high nucleic acid (HNA) bacterial cells in the affected river suggested the transformation of dormant to active cells due to the POME final discharge. This is the first study to report on the shift of LNA/HNA ratios which may serves as a potential bioindicator in the screening of the anthropogenic effects due to POME final discharge in river water with originally high LNA proportions. Monitoring the effluent discharge at low trophic level using flow cytometry is a rapid and sensitive approach when compared to the current physicochemical assessment method. This approach allows for the screening of river water contamination caused by POME final discharge prior to a full assessment using the recently proposed specific bacterial indicators. Elsevier 2018-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73883/1/PALM.pdf Sharuddin, Siti Suhailah and Ramli, Norhayati and Mohd Nor, Diana and Hassan, Mohd Ali and Maeda, Toshinari and Shirai, Yoshihito and Sakai, Kenji and Tashiro, Yukihiro (2018) Shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge. Ecological Indicators, 85. 79 - 84. ISSN 1470-160X https://www.sciencedirect.com/science/article/pii/S1470160X17306441 10.1016/j.ecolind.2017.10.020 |
| spellingShingle | Sharuddin, Siti Suhailah Ramli, Norhayati Mohd Nor, Diana Hassan, Mohd Ali Maeda, Toshinari Shirai, Yoshihito Sakai, Kenji Tashiro, Yukihiro Shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge |
| title | Shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge |
| title_full | Shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge |
| title_fullStr | Shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge |
| title_full_unstemmed | Shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge |
| title_short | Shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge |
| title_sort | shift of low to high nucleic acid bacteria as potential bioindicator for the screening of anthropogenic effects in the receiving river due to palm oil mill effluent final discharge |
| url | http://psasir.upm.edu.my/id/eprint/73883/ http://psasir.upm.edu.my/id/eprint/73883/ http://psasir.upm.edu.my/id/eprint/73883/ http://psasir.upm.edu.my/id/eprint/73883/1/PALM.pdf |