Purification and properties of polygalacturonase associated with the infection process of Colletotrichum truncatum CP2 in chilli
In this study, polygalacturonase enzyme produced by Colletotrichum truncatum CP2 was partially purified by aqueous two-phase system and the properties of this enzyme was characterized. The highest yield (57.4%) and purification fold (5.1) was obtained using 22% PEG 6,000/15% sodium citrate comprisin...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AD Publications
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/73683/ http://psasir.upm.edu.my/id/eprint/73683/1/Purification%20and%20properties%20of%20polygalacturonase%20associated%20with%20the%20infection%20process%20of%20Colletotrichum%20truncatum%20CP2%20in%20chilli.pdf |
| _version_ | 1848857335338893312 |
|---|---|
| author | Mohamad Remli, Nurul Atika Md Shah, Umi Kalsom Ariff, Arbakariya Yusof, Mohd Termizi Lim, Jeffrey Seng Heng |
| author_facet | Mohamad Remli, Nurul Atika Md Shah, Umi Kalsom Ariff, Arbakariya Yusof, Mohd Termizi Lim, Jeffrey Seng Heng |
| author_sort | Mohamad Remli, Nurul Atika |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, polygalacturonase enzyme produced by Colletotrichum truncatum CP2 was partially purified by aqueous two-phase system and the properties of this enzyme was characterized. The highest yield (57.4%) and purification fold (5.1) was obtained using 22% PEG 6,000/15% sodium citrate comprising crude load of 16% (w/w) at pH 7.0 with addition of 1.0% (w/w) sodium chloride. The partially purified PG remained active over a wide range of pH (2.5-6.0) and the optimum activity was obtained at pH 5.0. Incubation of the partially purified PG at 40 and 50 °C for 30 min caused the activity of PG to decrease up to 20% and 40%, respectively. However, no significant changes in the activity when the enzymes were incubated up to 4 h at 40 and 50 °C. The results from this study suggested that ATPS comprising of PEG and sodium citrate could be potentially used as an alternative method for purification of PG. |
| first_indexed | 2025-11-15T11:55:55Z |
| format | Article |
| id | upm-73683 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:55:55Z |
| publishDate | 2018 |
| publisher | AD Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-736832020-05-12T16:27:02Z http://psasir.upm.edu.my/id/eprint/73683/ Purification and properties of polygalacturonase associated with the infection process of Colletotrichum truncatum CP2 in chilli Mohamad Remli, Nurul Atika Md Shah, Umi Kalsom Ariff, Arbakariya Yusof, Mohd Termizi Lim, Jeffrey Seng Heng In this study, polygalacturonase enzyme produced by Colletotrichum truncatum CP2 was partially purified by aqueous two-phase system and the properties of this enzyme was characterized. The highest yield (57.4%) and purification fold (5.1) was obtained using 22% PEG 6,000/15% sodium citrate comprising crude load of 16% (w/w) at pH 7.0 with addition of 1.0% (w/w) sodium chloride. The partially purified PG remained active over a wide range of pH (2.5-6.0) and the optimum activity was obtained at pH 5.0. Incubation of the partially purified PG at 40 and 50 °C for 30 min caused the activity of PG to decrease up to 20% and 40%, respectively. However, no significant changes in the activity when the enzymes were incubated up to 4 h at 40 and 50 °C. The results from this study suggested that ATPS comprising of PEG and sodium citrate could be potentially used as an alternative method for purification of PG. AD Publications 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73683/1/Purification%20and%20properties%20of%20polygalacturonase%20associated%20with%20the%20infection%20process%20of%20Colletotrichum%20truncatum%20CP2%20in%20chilli.pdf Mohamad Remli, Nurul Atika and Md Shah, Umi Kalsom and Ariff, Arbakariya and Yusof, Mohd Termizi and Lim, Jeffrey Seng Heng (2018) Purification and properties of polygalacturonase associated with the infection process of Colletotrichum truncatum CP2 in chilli. International Journal of Environmental & Agriculture Research, 4 (8). pp. 1-9. ISSN 2454-1850 https://www.neliti.com/publications/262129/purification-and-properties-of-polygalacturonase-associated-with-the-infection-p |
| spellingShingle | Mohamad Remli, Nurul Atika Md Shah, Umi Kalsom Ariff, Arbakariya Yusof, Mohd Termizi Lim, Jeffrey Seng Heng Purification and properties of polygalacturonase associated with the infection process of Colletotrichum truncatum CP2 in chilli |
| title | Purification and properties of polygalacturonase associated with the infection process of Colletotrichum truncatum CP2 in chilli |
| title_full | Purification and properties of polygalacturonase associated with the infection process of Colletotrichum truncatum CP2 in chilli |
| title_fullStr | Purification and properties of polygalacturonase associated with the infection process of Colletotrichum truncatum CP2 in chilli |
| title_full_unstemmed | Purification and properties of polygalacturonase associated with the infection process of Colletotrichum truncatum CP2 in chilli |
| title_short | Purification and properties of polygalacturonase associated with the infection process of Colletotrichum truncatum CP2 in chilli |
| title_sort | purification and properties of polygalacturonase associated with the infection process of colletotrichum truncatum cp2 in chilli |
| url | http://psasir.upm.edu.my/id/eprint/73683/ http://psasir.upm.edu.my/id/eprint/73683/ http://psasir.upm.edu.my/id/eprint/73683/1/Purification%20and%20properties%20of%20polygalacturonase%20associated%20with%20the%20infection%20process%20of%20Colletotrichum%20truncatum%20CP2%20in%20chilli.pdf |