Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos
Objective: To investigate phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos. Methods: Fatty acid methyl ester was analyzed by gas chromatography-flame ionization detector. Antioxidant activity was determined using 2,2-diphenyl-p...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73485/ http://psasir.upm.edu.my/id/eprint/73485/1/AAPTOS.pdf |
| _version_ | 1848857283533996032 |
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| author | Mat Rashid, Zalilawati Ali, Abdul M. Douzenel, Philippe Bourgougnon, Nathalie Shaari, Khozirah Andriani, Yosie Tengku Muhammad, Tengku Sifzizul Mohamad, Habsah |
| author_facet | Mat Rashid, Zalilawati Ali, Abdul M. Douzenel, Philippe Bourgougnon, Nathalie Shaari, Khozirah Andriani, Yosie Tengku Muhammad, Tengku Sifzizul Mohamad, Habsah |
| author_sort | Mat Rashid, Zalilawati |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: To investigate phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos. Methods: Fatty acid methyl ester was analyzed by gas chromatography-flame ionization detector. Antioxidant activity was determined using 2,2-diphenyl-picrylhydrazyl radical scavenging assay and total phenolics content by Folin-Ciocalteu procedure. Vero cells viability was evaluated using methyl thiazole tetrazolium and the inactivation of herpes simplex virus type 1 by neutral red uptake assay. p-Hydroxybenzamide isolated by column chromatography was characterized by utilizing nuclear magnetic resonance spectroscopy and electron impact mass spectrometry. Results: The chloroform, ethyl acetate and methanol extracts of Aaptos aaptos produced higher portions of straight-chain saturated fatty acid, while hexane extract mainly consisted of unsaturated fatty acid. The five majors of fatty acid methyl ester were identified as behenic acid, cis-10-heptadecenoic acid and cis-10-pentadecenoic acids, palmitic acid and tricosanoic acid. In addition, among all organic extracts, chloroform extract inactivated herpes simplex virus type 1 while exhibited weak cytotoxic activity against normal Vero cells and also exhibited strong cytotoxic activity on HL-60, MCF-7, K562, CEM-SS and WEHI-3B cells. A phenolic compound, p-hydroxybenzamide was also isolated from the sponge. Conclusions: Aaptos aaptos could be a source to derive the potential antiviral and anticancer agents. However, further studies are needed to determine the mechanism involved in the process. |
| first_indexed | 2025-11-15T11:55:05Z |
| format | Article |
| id | upm-73485 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:55:05Z |
| publishDate | 2018 |
| publisher | Wolters Kluwer Medknow Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-734852021-07-15T07:19:03Z http://psasir.upm.edu.my/id/eprint/73485/ Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos Mat Rashid, Zalilawati Ali, Abdul M. Douzenel, Philippe Bourgougnon, Nathalie Shaari, Khozirah Andriani, Yosie Tengku Muhammad, Tengku Sifzizul Mohamad, Habsah Objective: To investigate phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos. Methods: Fatty acid methyl ester was analyzed by gas chromatography-flame ionization detector. Antioxidant activity was determined using 2,2-diphenyl-picrylhydrazyl radical scavenging assay and total phenolics content by Folin-Ciocalteu procedure. Vero cells viability was evaluated using methyl thiazole tetrazolium and the inactivation of herpes simplex virus type 1 by neutral red uptake assay. p-Hydroxybenzamide isolated by column chromatography was characterized by utilizing nuclear magnetic resonance spectroscopy and electron impact mass spectrometry. Results: The chloroform, ethyl acetate and methanol extracts of Aaptos aaptos produced higher portions of straight-chain saturated fatty acid, while hexane extract mainly consisted of unsaturated fatty acid. The five majors of fatty acid methyl ester were identified as behenic acid, cis-10-heptadecenoic acid and cis-10-pentadecenoic acids, palmitic acid and tricosanoic acid. In addition, among all organic extracts, chloroform extract inactivated herpes simplex virus type 1 while exhibited weak cytotoxic activity against normal Vero cells and also exhibited strong cytotoxic activity on HL-60, MCF-7, K562, CEM-SS and WEHI-3B cells. A phenolic compound, p-hydroxybenzamide was also isolated from the sponge. Conclusions: Aaptos aaptos could be a source to derive the potential antiviral and anticancer agents. However, further studies are needed to determine the mechanism involved in the process. Wolters Kluwer Medknow Publications 2018-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73485/1/AAPTOS.pdf Mat Rashid, Zalilawati and Ali, Abdul M. and Douzenel, Philippe and Bourgougnon, Nathalie and Shaari, Khozirah and Andriani, Yosie and Tengku Muhammad, Tengku Sifzizul and Mohamad, Habsah (2018) Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos. Asian Pacific Journal of Tropical Biomedicine, 8 (11). 554 - 564. ISSN 2221-1691; ESSN: 2588-9222 https://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=11;spage=554;epage=564;aulast=Rashid;type=0 10.4103/2221-1691.245971 |
| spellingShingle | Mat Rashid, Zalilawati Ali, Abdul M. Douzenel, Philippe Bourgougnon, Nathalie Shaari, Khozirah Andriani, Yosie Tengku Muhammad, Tengku Sifzizul Mohamad, Habsah Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos |
| title | Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos |
| title_full | Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos |
| title_fullStr | Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos |
| title_full_unstemmed | Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos |
| title_short | Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos |
| title_sort | phenolics, fatty acids composition and biological activities of various extracts and fractions of malaysian aaptos aaptos |
| url | http://psasir.upm.edu.my/id/eprint/73485/ http://psasir.upm.edu.my/id/eprint/73485/ http://psasir.upm.edu.my/id/eprint/73485/ http://psasir.upm.edu.my/id/eprint/73485/1/AAPTOS.pdf |