Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor
Diacylglycerol (DAG) and triacylglycerol (TAG) as responses on optimization of DAG production using a dual response approach of response surface methodology were investigated. This approach takes the molecular equilibrium of DAG into account and allows for the optimization of reaction conditions to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
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American Chemical Society
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7303/ http://psasir.upm.edu.my/id/eprint/7303/1/Diacylglycerol%20and%20Triacylglycerol%20as%20Responses%20in%20a%20Dual%20Response%20Surface.pdf |
| _version_ | 1848840557383647232 |
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| author | Lo, Seong Koon Cheong, Ling Zhi Arifin, Norlelawati Tan, Chin Ping Long, Kamariah Yusoff, Mohd Suria Affandi Lai, Oi Ming |
| author_facet | Lo, Seong Koon Cheong, Ling Zhi Arifin, Norlelawati Tan, Chin Ping Long, Kamariah Yusoff, Mohd Suria Affandi Lai, Oi Ming |
| author_sort | Lo, Seong Koon |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Diacylglycerol (DAG) and triacylglycerol (TAG) as responses on optimization of DAG production using a dual response approach of response surface methodology were investigated. This approach takes the molecular equilibrium of DAG into account and allows for the optimization of reaction conditions to achieve maximum DAG and minimum TAG yields. The esterification reaction was optimized with four factors using a central composite rotatable design. The following optimized conditions yielded 48 wt % DAG and 14 wt % TAG: reaction temperature of 66.29 °C, enzyme dosage of 4 wt %, fatty acid/glycerol molar ratio of 2.14, and reaction time of 4.14 h. Similar results were achieved when the process was scaled up to a 10 kg production in a pilot packed-bed enzyme reactor. Lipozyme RM IM did not show any significant activity losses or changes in fatty acid selectivity on DAG synthesis during the 10 pilot productions. However, lipozyme RM IM displayed higher selectivity toward the production of oleic acid-enriched DAG. The purity of DAG oil after purification was 92 wt %. |
| first_indexed | 2025-11-15T07:29:14Z |
| format | Article |
| id | upm-7303 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:29:14Z |
| publishDate | 2007 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-73032015-10-05T08:33:38Z http://psasir.upm.edu.my/id/eprint/7303/ Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor Lo, Seong Koon Cheong, Ling Zhi Arifin, Norlelawati Tan, Chin Ping Long, Kamariah Yusoff, Mohd Suria Affandi Lai, Oi Ming Diacylglycerol (DAG) and triacylglycerol (TAG) as responses on optimization of DAG production using a dual response approach of response surface methodology were investigated. This approach takes the molecular equilibrium of DAG into account and allows for the optimization of reaction conditions to achieve maximum DAG and minimum TAG yields. The esterification reaction was optimized with four factors using a central composite rotatable design. The following optimized conditions yielded 48 wt % DAG and 14 wt % TAG: reaction temperature of 66.29 °C, enzyme dosage of 4 wt %, fatty acid/glycerol molar ratio of 2.14, and reaction time of 4.14 h. Similar results were achieved when the process was scaled up to a 10 kg production in a pilot packed-bed enzyme reactor. Lipozyme RM IM did not show any significant activity losses or changes in fatty acid selectivity on DAG synthesis during the 10 pilot productions. However, lipozyme RM IM displayed higher selectivity toward the production of oleic acid-enriched DAG. The purity of DAG oil after purification was 92 wt %. American Chemical Society 2007-06-16 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7303/1/Diacylglycerol%20and%20Triacylglycerol%20as%20Responses%20in%20a%20Dual%20Response%20Surface.pdf Lo, Seong Koon and Cheong, Ling Zhi and Arifin, Norlelawati and Tan, Chin Ping and Long, Kamariah and Yusoff, Mohd Suria Affandi and Lai, Oi Ming (2007) Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor. Journal of Agricultural and Food Chemistry, 55 (14). pp. 5595-5603. ISSN 0021-8561 http://dx.doi.org/10.1021/jf0706676 10.1021/jf0706676 English |
| spellingShingle | Lo, Seong Koon Cheong, Ling Zhi Arifin, Norlelawati Tan, Chin Ping Long, Kamariah Yusoff, Mohd Suria Affandi Lai, Oi Ming Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor |
| title | Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor |
| title_full | Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor |
| title_fullStr | Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor |
| title_full_unstemmed | Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor |
| title_short | Diacylglycerol and Triacylglycerol as Responses in a Dual Response Surface-Optimized Process for Diacylglycerol Production by Lipase-Catalyzed Esterification in a Pilot Packed-Bed Enzyme Reactor |
| title_sort | diacylglycerol and triacylglycerol as responses in a dual response surface-optimized process for diacylglycerol production by lipase-catalyzed esterification in a pilot packed-bed enzyme reactor |
| url | http://psasir.upm.edu.my/id/eprint/7303/ http://psasir.upm.edu.my/id/eprint/7303/ http://psasir.upm.edu.my/id/eprint/7303/ http://psasir.upm.edu.my/id/eprint/7303/1/Diacylglycerol%20and%20Triacylglycerol%20as%20Responses%20in%20a%20Dual%20Response%20Surface.pdf |