Enhancement of a protocol purifying T1 lipase through molecular approach

Enhancement of a protocol purifying T1 lipase through molecular approach

T1 Lipase is a thermostable secretary protein of Geobacillus zalihae strain previously expressed in a prokaryotic system and purified using three-step purification: affinity 1, affinity 2, and ion exchange chromatography (IEX). This approach is time consuming and offers low purity and recovery yield...

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Bibliographic Details
Main Authors: Che Hussian, Che Haznie Ayu, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri
Format: Article
Language:English
Published: PeerJ 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72578/
http://psasir.upm.edu.my/id/eprint/72578/1/Enhancement%20of%20a%20protocol%20purifying%20T1%20lipase%20through%20molecular%20approach.pdf

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