Enhancement of a protocol purifying T1 lipase through molecular approach
T1 Lipase is a thermostable secretary protein of Geobacillus zalihae strain previously expressed in a prokaryotic system and purified using three-step purification: affinity 1, affinity 2, and ion exchange chromatography (IEX). This approach is time consuming and offers low purity and recovery yield...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72578/ http://psasir.upm.edu.my/id/eprint/72578/1/Enhancement%20of%20a%20protocol%20purifying%20T1%20lipase%20through%20molecular%20approach.pdf |