Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay
Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease. Differentiation of the virus into virulent and avirulent is necessary for effective control of the disease. Biopanning experiments were performed using a disulfide constrained pha...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
2009 Elsevier B.V.
2006
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/7116/ http://psasir.upm.edu.my/id/eprint/7116/1/Detection%20of%20virulent%20Newcastle%20disease%20virus%20using%20a%20phage.pdf |
| _version_ | 1848840506286538752 |
|---|---|
| author | Lee, Thong Chuan Yusoff, Khatijah Nathan, Sheila Tan, Wen Siang |
| author_facet | Lee, Thong Chuan Yusoff, Khatijah Nathan, Sheila Tan, Wen Siang |
| author_sort | Lee, Thong Chuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease. Differentiation of the virus into virulent and avirulent is necessary for effective control of the disease. Biopanning experiments were performed using a disulfide constrained phage displayed heptapeptide library against three pathotypes of NDV strains: velogenic (highly virulent), mesogenic (moderately virulent) and lentogenic (avirulent). A phage clone bearing the peptide sequence SWGEYDM capable of distinguishing virulent from avirulent NDV strains was isolated. This phage clone was employed as a diagnostic reagent in a dot blot assay and it successfully detected only virulent NDV strains.
|
| first_indexed | 2025-11-15T07:28:25Z |
| format | Article |
| id | upm-7116 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:28:25Z |
| publishDate | 2006 |
| publisher | 2009 Elsevier B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-71162015-10-05T08:45:55Z http://psasir.upm.edu.my/id/eprint/7116/ Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay Lee, Thong Chuan Yusoff, Khatijah Nathan, Sheila Tan, Wen Siang Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease. Differentiation of the virus into virulent and avirulent is necessary for effective control of the disease. Biopanning experiments were performed using a disulfide constrained phage displayed heptapeptide library against three pathotypes of NDV strains: velogenic (highly virulent), mesogenic (moderately virulent) and lentogenic (avirulent). A phage clone bearing the peptide sequence SWGEYDM capable of distinguishing virulent from avirulent NDV strains was isolated. This phage clone was employed as a diagnostic reagent in a dot blot assay and it successfully detected only virulent NDV strains. 2009 Elsevier B.V. 2006-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7116/1/Detection%20of%20virulent%20Newcastle%20disease%20virus%20using%20a%20phage.pdf Lee, Thong Chuan and Yusoff, Khatijah and Nathan, Sheila and Tan, Wen Siang (2006) Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay. Journal of Virological Methods, 136 (1-2). pp. 224-229. ISSN 0166-0934 http://dx.doi.org/10.1016/j.jviromet.2006.05.017 10.1016/j.jviromet.2006.05.017 English |
| spellingShingle | Lee, Thong Chuan Yusoff, Khatijah Nathan, Sheila Tan, Wen Siang Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay |
| title | Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay |
| title_full | Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay |
| title_fullStr | Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay |
| title_full_unstemmed | Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay |
| title_short | Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay |
| title_sort | detection of virulent newcastle disease virus using a phage-capturing dot blot assay |
| url | http://psasir.upm.edu.my/id/eprint/7116/ http://psasir.upm.edu.my/id/eprint/7116/ http://psasir.upm.edu.my/id/eprint/7116/ http://psasir.upm.edu.my/id/eprint/7116/1/Detection%20of%20virulent%20Newcastle%20disease%20virus%20using%20a%20phage.pdf |